Impaired Differentiation of Langerhans Cells in the Murine Oral Epithelium Adjacent to Titanium Dental Implants

Frontiers in Immunology
Oded HeymanAsaf Wilensky

Abstract

Peri-implantitis is a destructive inflammatory process affecting tissues surrounding dental implants and it is considered a new global health concern. Human studies have suggested that the frequencies of Langerhans cells (LCs), the main antigen-presenting cells (APCs) of the oral epithelium, are dysregulated around the implants. Since LCs play a role in regulating oral mucosal homeostasis, we studied the impact of dental titanium implants on LC differentiation using a novel murine model. We demonstrate that whereas the percentage of LC precursors (CD11c+MHCII+) increased in the peri-implant epithelium, the frequencies of LCs (CD11c+MHCII+EpCAM+langerin+) were significantly reduced. Instead, a population of partially developed LCs expressing CD11c+MHCII+EpCAM+ but not langerin evolved in the peri-implant mucosa, which was also accompanied by a considerable leukocyte infiltrate. In line with the increased levels of LC precursors, expression of CCL2 and CCL20, chemokines mediating their translocation to the epithelium, was elevated in the peri-implant epithelium. However, expression of TGF-β1, the major cytokine driving final differentiation of LCs, was reduced in the epithelium. Further analysis revealed that while the expression...Continue Reading

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Citations

Aug 28, 2020·Journal of Periodontology·Georgios A KotsakisDiane Daubert
Sep 10, 2020·Clinical Implant Dentistry and Related Research·Pravej SerichetaphongseAtiphan Pimkhaokham
Sep 30, 2020·Journal of Clinical Periodontology·Xue YuanJill A Helms
Dec 31, 2020·BioMed Research International·Milad BaseriHossein Samadi Kafil
Apr 27, 2021·Journal of Dental Research·H SharawiA Wilensky
Jun 5, 2021·Trends in Immunology·Khaled Zubeidat, Avi-Hai Hovav

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Datasets Mentioned

BETA
GM-CSF

Methods Mentioned

BETA
flow cytometry
FCS
PCR
FACS

Software Mentioned

Image J
FlowJo
ImageJ

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