Impaired expression of the mitochondrial calcium uniporter suppresses mast cell degranulation

Molecular and Cellular Biochemistry
Tadahide FurunoMamoru Nakanishi

Abstract

Calcium ion (Ca(2+)) uptake into the mitochondrial matrix influences ATP production, Ca(2+) homeostasis, and apoptosis regulation. Ca(2+) uptake across the ion-impermeable inner mitochondrial membrane is mediated by the mitochondrial Ca(2+) uniporter (MCU) complex. The MCU complex forms a pore structure composed of several proteins. MCU is a Ca(2+)-selective channel in the inner-mitochondrial membrane that allows electrophoretic Ca(2+) entry into the matrix. Mitochondrial Ca(2+) uptake 1 (MICU1) functions as a Ca(2+)-sensing regulator of the MCU complex. Previously, by microscopic analysis at the single-cell level, we found that during mast cell activation, mitochondria capture cytosolic Ca(2+) in two steps. Consequently, mitochondrial Ca(2+) uptake likely plays a role in cellular function through cytosolic Ca(2+) buffering. Here, we investigate the role of MCU and MICU1 in mitochondrial Ca(2+) uptake and mast cell degranulation using MCU- and MICU1-knockdown (KD) mast cells. Whereas MCU- and MICU1-KD mast cells show normal proliferation rates and mitochondrial membrane potential, they exhibit slow and reduced cytosolic and mitochondrial Ca(2+) elevation after antigen stimulation. Moreover, β-hexosaminidase release induced by a...Continue Reading

References

Nov 5, 2002·Current Opinion in Immunology·Juan Rivera
Mar 18, 2005·Annual Review of Immunology·Stephen J GalliMindy Tsai
Jul 4, 2006·Biochemical and Biophysical Research Communications·Rika NakamuraMamoru Nakanishi
Aug 10, 2010·Nature·Fabiana PerocchiVamsi K Mootha
Jul 24, 2012·Proceedings of the National Academy of Sciences of the United States of America·Ilaria DragoTullio Pozzan
Aug 7, 2012·Biological & Pharmaceutical Bulletin·Marie TakekawaMamoru Nakanishi
Nov 28, 2012·Nature Cell Biology·Karthik MallilankaramanMuniswamy Madesh
Jun 14, 2013·Proceedings of the National Academy of Sciences of the United States of America·George S B WilliamsW J Lederer
Mar 19, 2014·Cell Calcium·Diana PendinTullio Pozzan
May 13, 2014·Biochemical and Biophysical Research Communications·Kimberli J KamerVamsi K Mootha
Jun 7, 2015·Cell Calcium·Marta Murgia, Rosario Rizzuto

❮ Previous
Next ❯

Citations

Aug 21, 2019·Journal of Applied Toxicology : JAT·Juyoung K ShimJulie A Gosse
Mar 5, 2016·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Dang Van CuongJin Han
Jul 26, 2018·Frontiers in Cell and Developmental Biology·Satoru YokawaTadahide Furuno
Apr 29, 2016·American Journal of Physiology. Cell Physiology·Bong Sook JhunJin O-Uchi
Mar 6, 2017·Biochemistry. Biokhimii︠a︡·M A ChelombitkoB V Chernyak
Nov 13, 2020·Frontiers in Immunology·Maria A ChelombitkoLakhsmi Bhargavi Paruchuru
Nov 23, 2017·Metabolism: Clinical and Experimental·Senta M KapnickPeter J McGuire

❮ Previous
Next ❯

Related Concepts

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis