Impaired Hepatic Vitamin A Metabolism in NAFLD Mice Leading to Vitamin A Accumulation in Hepatocytes.

Cellular and Molecular Gastroenterology and Hepatology
Ali SaeedKlaas Nico Faber

Abstract

Systemic retinol (vitamin A) homeostasis is controlled by the liver, involving close collaboration between hepatocytes and hepatic stellate cells (HSCs). Genetic variants in retinol metabolism (PNPLA3 and HSD17B13) are associated with non-alcoholic fatty liver disease (NAFLD) and disease progression. Still, little mechanistic details are known about hepatic vitamin A metabolism in NAFLD, which may affect carbohydrate and lipid metabolism, inflammation, oxidative stress and the development of fibrosis and cancer, e.g. all risk factors of NAFLD. Here, we analyzed vitamin A metabolism in 2 mouse models of NAFLD; mice fed a high-fat, high-cholesterol (HFC) diet and Leptinob mutant (ob/ob) mice. Hepatic retinol and retinol binding protein 4 (RBP4) levels were significantly reduced in both mouse models of NAFLD. In contrast, hepatic retinyl palmitate levels (the vitamin A storage form) were significantly elevated in these mice. Transcriptome analysis revealed a hyperdynamic state of hepatic vitamin A metabolism, with enhanced retinol storage and metabolism (upregulated Lrat, Dgat1, Pnpla3, Raldh's and RAR/RXR-target genes) in fatty livers, in conjunction with induced hepatic inflammation (upregulated Cd68, Tnfα, Nos2, Il1β, Il-6) and...Continue Reading

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Citations

Jan 27, 2021·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Baixue LiJames L Boyer
Jul 6, 2021·World Journal of Clinical Cases·Fu-Chen YangGuo-Xun Chen
Jul 6, 2021·Liver Research·Sana RazaRohit A Sinha

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Methods Mentioned

BETA
density gradient centrifugation
protein assay

Software Mentioned

Image Lab
ImageJ
GraphPad
Primer Express
GraphPad Prism
Aperio ImageScope

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