PMID: 9168404May 1, 1997Paper

Impaired induction of the apoptosis-protective protein Bcl-xL in activated PBMC from asymptomatic HIV-infected individuals

Journal of Clinical Immunology
P J BlairC H June

Abstract

Progression to AIDS in asymptomatic HIV-infected individuals is characterized by a gradual but progressive loss of CD4+ T cells. While the mechanisms underlying this decline are currently unknown, recent evidence suggests that these cells are abnormally sensitive to apoptosis in response to activation signals. Recent work has implicated downregulation of Bcl-2 with the increased spontaneous apoptosis in lymphocytes from HIV-infected patients. We have evaluated the roles of the apoptosis-protective proteins Bcl-2 and Bcl-x in stimulated PBMC from asymptomatic HIV-infected and HIV-uninfected individuals. We found that Bcl-2 was constitutively expressed in PBMC from both HIV-infected and uninfected samples. However, Bcl-x induction was delayed and responses were decreased in stimulated HIV-infected samples. Additionally, single-cell intracellular staining of Bcl-x revealed a significant inverse correlation between PWM-induced Bcl-x expression and apoptosis (r = -0.695, P = 0.05). This was confirmed at the single-cell level in direct experiments when stimulated cells were sorted based on Bcl-x induction and then measured for apoptosis. Furthermore, low Bcl-x expression was not due to reduced lymphocyte activation following PWM stim...Continue Reading

Citations

Apr 13, 2010·Retrovirology·Georges HerbeinWasim Abbas
Jun 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Julie A CookAndrew J Henderson
Mar 23, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Constantinos PetrovasPeter D Katsikis
Aug 30, 2013·The Open Virology Journal·Wasim Abbas, Georges Herbein
Aug 21, 2008·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·Syh-Jae LinMing-Ling Kuo
Sep 25, 1999·Clinical Immunology : the Official Journal of the Clinical Immunology Society·S J ChavanS G Pahwa
Jul 8, 2000·Kidney International·A Ortiz

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