Impaired myelopoiesis in mice devoid of interferon regulatory factor 1

Leukemia
U TestaA Battistini

Abstract

Interferon regulatory factor (IRF)-1 is a transcription factor controlling the expression of several genes, which are differentially induced depending on the cell type and signal. IRF-1 modulates multiple functions, including regulation of immune responses and host defence, cell growth, cytokine signalling and hematopoietic development. Here, we investigated the role of IRF-1 in granulocytic differentiation in mice with a null mutation in the IRF-1 gene. We show that IRF-1(-/-) bone marrow cells exhibit an increased number of immature granulocytic precursors, associated with a decreased number of mature granulocytic elements as compared to normal mice, suggestive of a defective maturation process. Clonogenetic analyses revealed a reduced number of CFU-G, CFU-M and CFU-GM colonies in IRF-1(-/-) mice, while the number of BFU-E/CFU-E colonies was unchanged. At the molecular level, the expression of CAAT-enhancer-binding protein (C/EBP)-epsilon, -alpha and PU.1 was substantially lower in the CD11b(+) cells from the bone marrow of IRF-1(-/-) mice as compared to cells from wild-type mice. These results, together with the fact that IRF-1 is markedly induced early during granulo-monocytic differentiation of CD34+ cells, highlight the p...Continue Reading

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Citations

Jan 6, 2010·Cancer Immunology, Immunotherapy : CII·David SavitskyTadatsugu Taniguchi
Nov 26, 2009·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Angela Battistini
Mar 24, 2011·International Immunology·Yana Khalfin-RabinovichBen-Zion Levi
Feb 29, 2008·Annual Review of Immunology·Tomohiko TamuraTadatsugu Taniguchi
Mar 26, 2013·Cellular and Molecular Life Sciences : CMLS·René HuberKorbinian Brand
Jan 17, 2008·Cells, Tissues, Organs·Ralf SchwanbeckUrsula Just
May 9, 2014·Biochimica Et Biophysica Acta·Guang-Nian ZhaoHongliang Li
Aug 25, 2010·Blood·Jacqueline BoultwoodJames S Wainscoat
Mar 31, 2015·PloS One·Flavia BiamonteFrancesco Costanzo
Dec 15, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Katherine C MacNamaraGary M Winslow
Jul 22, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Alessandra FragaleAngela Battistini

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