Impaired platelet activation and cAMP homeostasis in MRP4-deficient mice.

Blood
Benoit DecouturePascale Gaussem

Abstract

Molecules that reduce the level of cyclic adenosine 5'-monophosphate (cAMP) in the platelet cytosol, such as adenosine 5'-diphosphate (ADP) secreted from dense granules, trigger platelet activation. Therefore, any change in the distribution and/or availability of cyclic nucleotides or ADP may interfere with platelet reactivity. In this study, we evaluated the role of multidrug resistance protein 4 (MRP4, or ABCC4), a nucleotide transporter, in platelet functions in vivo and in vitro by investigating MRP4-deficient mice. MRP4 deletion resulted in a slight increase in platelet count but had no impact on platelet ultrastructure. In MRP4-deficient mice, the arterial occlusion was delayed and the tail bleeding time was prolonged. In a model of platelet depletion and transfusion mimicking a platelet-specific knockout, mice injected with MRP4(-/-) platelets also showed a significant increase in blood loss compared with mice injected with wild-type platelets. Defective thrombus formation and platelet activation were confirmed in vitro by studying platelet adhesion to collagen in flow conditions, integrin αIIbβ3 activation, washed platelet secretion, and aggregation induced by low concentrations of proteinase-activated receptor 4-activa...Continue Reading

References

Jul 13, 2000·The Journal of Biological Chemistry·G JedlitschkyD Keppler
Aug 18, 2004·Molecular and Cellular Biology·Markos LeggasJohn D Schuetz
Mar 21, 2008·Trends in Pharmacological Sciences·Frans G M RusselRosalinde Masereeuw
Apr 1, 2008·Proceedings of the National Academy of Sciences of the United States of America·Keisuke SawadaYoshinori Moriyama
May 20, 2008·Journal of Thrombosis and Haemostasis : JTH·S GambaryanU Walter
Jan 15, 2009·International Journal of Cancer. Journal International Du Cancer·Lena OevermannChristoph A Ritter
Feb 4, 2010·Circulation·Paul A Gurbel, Udaya S Tantry
Feb 6, 2010·The American Journal of Pathology·Gabriele JedlitschkyAndreas Greinacher
Jun 15, 2011·The Journal of Clinical Investigation·Yannis HaraJean-Sébastien Hulot
Jul 28, 2011·Purinergic Signalling·Béatrice Hechler, Christian Gachet
Nov 18, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yassine SassiJean-Sébastien Hulot
Dec 6, 2011·Journal of Thrombosis and Haemostasis : JTH·A Smolenski
Sep 18, 2012·Experimental Hematology·Antonija Jurak BegonjaUlrich Walter
Sep 28, 2012·Thrombosis and Haemostasis·Alessandra Borgognone, Fabio Maria Pulcinelli
Oct 18, 2012·Annual Review of Pharmacology and Toxicology·Satish CheepalaJohn D Schuetz
Oct 1, 2013·Journal of the American College of Cardiology·Udaya S TantryUNKNOWN Working Group on On-Treatment Platelet Reactivity
Apr 23, 2014·PloS One·Shailesh R AgarwalRobert D Harvey
May 20, 2014·European Journal of Pharmacology·Li-Ming LienKuan-Hung Lin
Jun 7, 2014·British Journal of Clinical Pharmacology·Isabella MassimiFabio M Pulcinelli
Aug 15, 2014·Pharmacogenetics and Genomics·Susanne BröderdorfGabriele Jedlitschky

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Citations

Apr 12, 2016·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Tiphaine Belleville-RollandChristilla Bachelot-Loza
Apr 7, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Toni Schumacher, Ralf A Benndorf
Jun 24, 2017·Thrombosis Research·Wei WangYing Wang
Jul 18, 2018·Journal of Cardiovascular Medicine·Giulio La RosaFilippo Crea
Mar 22, 2018·F1000Research·Anish Sharda, Robert Flaumenhaft
Jun 30, 2019·Journal of Cell Science·C A I AlonsoS Perez-Martinez
Aug 16, 2018·The Journal of Pharmacology and Experimental Therapeutics·Gabriela Maria BertollottoFabiola Zakia Mónica
Jan 11, 2020·The Journal of Small Animal Practice·P GantK Humm
Apr 6, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jinxue ZhangYuan Zhang
Mar 3, 2020·Clinical Pharmacology and Therapeutics·Sanjay K NigamJeremiah D Momper
Nov 17, 2016·Platelets·Andrea L Ambrosio, Santiago M Di Pietro
Jun 16, 2017·Purinergic Signalling·Yoshinori MoriyamaMasatoshi Nomura
Aug 8, 2020·Journal of Clinical Medicine·Arnaud DupuisChristian Gachet
Jul 27, 2018·Research and Practice in Thrombosis and Haemostasis·Isabella MassimiFabio M Pulcinelli
Aug 24, 2019·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Benoit DecoutureChristilla Bachelot-Loza
Apr 15, 2021·Thrombosis and Haemostasis·Tiphaine Belleville-RollandChristilla Bachelot-Loza
May 10, 2021·Protein and Peptide Letters·Ioannis AngelisVassilios Tsikaris

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