Impaired RNA splicing of 5'-regulatory sequences of the astroglial glutamate transporter EAAT2 in human astrocytoma

Journal of Neurology, Neurosurgery, and Psychiatry
C MünchT Meyer

Abstract

A loss of the glutamate transporter EAAT2 has been reported in the neoplastic transformation of astrocytic cells and astrocytoma. The RNA expression of EAAT2 and five 5'-regulatory splice variants was investigated to identify alterations of the post-transcriptional EAAT2 gene regulation in human astrocytic tumours. Three known (EAAT2, HBGTII, and HBGTIIC) and two novel (EAAT2/3 and EAAT2/31) EAAT2 transcripts originating from alternative splicing of 5'-regulatory sequences were subject to an RNA expression analysis using reverse transcription and competitive PCR. Specimens of astrocytoma World Health Organisation (WHO) grade I-IV in 14 patients and control brain tissue obtained from three normal persons were studied. The main EAAT2 RNA was found to be equally expressed in normal human brain and astrocytic tumour samples. By contrast, the expression pattern of four 5'-variants of the transporter transcript was altered in the investigated series of astrocytoma compared with normal brain. HBGTII, HBGTIIC, and EAAT2/3 were amplified from seven and four tumours and one sample, respectively. EAAT2/31 was expressed in none of the tumour specimens studied. In conclusion, in astrocytic tumours of different histopathological grades there...Continue Reading

Citations

Apr 2, 2004·Neurobiology of Disease·Nicholas J Maragakis, Jeffrey D Rothstein
Apr 22, 2014·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·Simon V LiubinasAndrew H Kaye
Nov 10, 2015·Journal of the History of the Behavioral Sciences·Milan Zafirovski
Jan 20, 2004·Glia·Nicholas J MaragakisJeffrey D Rothstein
Nov 8, 2006·British Journal of Pharmacology·P M Beart, R D O'Shea
Jan 22, 2005·Brain Research. Molecular Brain Research·Joanna Rozyczka, Jürgen Engele
Oct 9, 2002·Oncogene·Hassan M Fathallah-ShaykhElia DiSavino
Nov 20, 2014·European Journal of Human Genetics : EJHG·Alessia FiorentinoAndrew McQuillin
Dec 2, 2006·The Journal of Biological Chemistry·Guilian TianChien-Liang Glenn Lin
Nov 14, 2007·Neurochemical Research·Christoph MünchThomas Meyer

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