Impaired spatial learning related with decreased expression of calcium/calmodulin-dependent protein kinase IIalpha and cAMP-response element binding protein in the pentylenetetrazol-kindled rats

Brain Research
Pei WangYue-Hui Fan

Abstract

Although its effect on cognitive functions has been one of the hot topics in the present neuroscience research, the mechanism of epilepsy related cognitive impairment is not clearly revealed. Intracellular Ca(2+) plays an important role in regulating many cellular functions including learning and memory, this experiment was therefore conducted, in which, we observed the behaviors of chronic epileptic rats kindled by pentylenetetrazol (PTZ) through Morris water maze (MWM), examined the concentration of intracellular free calcium ([Ca(2+)](i)) with flow cytometry, and tested the expression of calcium/calmodulin-dependent protein kinase IIalpha (CaMKII(alpha)) and cAMP-response element binding protein (CREB) in hippocampus of those rats using western blot and reverse transcription-polymerase chain reaction (RT-PCR). The results demonstrate impaired ability of spatial learning and memory, increased concentration of [Ca(2+)](i), decreased expression levels of total CaMKII(alpha), phosphorylated CaMKII(alpha) (P-CaMKII(alpha)) and phosphorylated CREB (P-CREB) and decreased levels of CaMKII(alpha) mRNA and CREB mRNA of the epileptic rats compared with the normal control rats. Moreover, Nimodipine, an inhibitor of voltage-dependent L-t...Continue Reading

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Citations

Apr 12, 2011·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Li-Jing JiaRui-Sheng Duan
Aug 25, 2012·Evidence-based Complementary and Alternative Medicine : ECAM·Ming Wai HungSimon Ming Yuen Lee
Feb 18, 2011·British Journal of Pharmacology·Yosef SarneOra Keren
May 9, 2018·Neurobiology of Disease·Evan C RosenbergFrances E Jensen

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