Impaired steroidogenesis in the testis of leptin-deficient mice (ob/ob -/-)

Acta histochemica
Fabiane Ferreira MartinsCarlos Alberto Mandarim-de-Lacerda

Abstract

The obesity and its comorbidities, including resistance to leptin, impacts the reproductive function. Testes express leptin receptors in the germ cells and Leydig cells. Then, leptin-deficient animals are obese and infertile. We aimed to evaluate the structure and steroidogenic pathway of the testis of deficient leptin mice. Three months old male C57BL/6 mice (wild-type, WT) and deficient leptin (ob/ob) mice had their testes dissected and prepared for analyses. Compared to the WT group, the ob/ob group showed a greater body mass with smaller testes, and alterations in the germinative epithelium: fewer spermatogonia, spermatocytes, and spermatids. The Sertoli cells and the germ cells showed condensed nuclei and nuclear fragmentation indicating cell death, in agreement with a low expression of the proliferating cell nuclear antigen and a high expression of Caspase3. In the ob/ob group, the sperm was absent in the seminiferous tubules, and the steroidogenic pathway was compromised (low 3Beta hydroxysteroid dehydrogenase and steroidogenic acute regulatory protein). Further, all hormone receptors involved in the testicular function were down expressed (androgen, estrogen, follicle-stimulating, luteinizing, aromatase, and nicotinamid...Continue Reading

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Citations

Nov 22, 2017·Journal of Cellular and Molecular Medicine·Antonio Pérez-PérezVíctor Sánchez-Margalet
Aug 26, 2020·Journal of Assisted Reproduction and Genetics·Nilgün YersalPetek Korkusuz
Jul 5, 2018·International Journal of Endocrinology·Yaelle ElfassyRachel Levy
Jun 26, 2020·International Journal of Molecular Sciences·Malgorzata BrzoskwiniaBarbara Bilinska
Apr 7, 2020·Molecular Reproduction and Development·Lalrawngbawli AnnieVikas Kumar Roy
Nov 14, 2020·Journal of Pediatric Endocrinology & Metabolism : JPEM·Isabel Viola WagnerOlle Söder
Jan 6, 2019·The American Journal of Pathology·Salvatore PanzaStefania Catalano

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