Impaired translation of CCAAT/enhancer binding protein alpha mRNA in bronchial smooth muscle cells of asthmatic patients

The Journal of Allergy and Clinical Immunology
Peter BorgerMichael Roth

Abstract

Bronchial smooth muscle (BSM) cells of asthmatic patients have an impaired expression of CCAAT/enhancer binding protein (C/EBP) alpha, which is associated with increased proliferation. We sought to assess the translational regulation of CEBPA mRNA in cultured BSM cells of healthy control subjects (n = 11) and asthmatic patients (n = 12). Translation efficiency was studied by using a translation control reporter system driven by the control elements present in the CEBPA mRNA. Translation efficiency was determined by the ratio of 2 artificial hemagglutinin (HA.11) proteins: p23 and p12. We also analyzed levels of proteins that control translation of CEBPA mRNA, namely heterogeneous nuclear ribonucleoprotein E2, calreticulin, eukaryotic translation initiation factor (eIF4E), and 4E binding protein. Compared with healthy control subjects, BSM cells of asthmatic patients proliferate faster (2.1-fold) and are primed for IL-6 secretion. Real-time RT-PCR showed that BSM cells of asthmatic patients express normal levels of CEBPA mRNA, whereas they express lower levels of C/EBPalpha (p42). Transient transfections with the translation control reporter system construct showed a disturbed p12/p23 ratio in BSM cells of asthmatic patients rel...Continue Reading

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Citations

May 13, 2006·American Journal of Respiratory and Critical Care Medicine·Peter BorgerMichael Roth
Nov 3, 2011·The Journal of Endocrinology·Abraham B Roos, Magnus Nord
Apr 23, 2013·Mediators of Inflammation·Petra Seidel, Michael Roth
Apr 14, 2012·Journal of Allergy·Nicola MiglinoPeter Borger
May 29, 2012·Pulmonary Pharmacology & Therapeutics·Yuxiu C XiaRachel L Clifford
May 15, 2009·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·J L Black, M Roth
Nov 24, 2011·American Journal of Respiratory Cell and Molecular Biology·Pamela J PlantJane Batt
Sep 14, 2018·Journal of Healthcare Engineering·Wenchao TangKe Ning
May 26, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·P SeidelM Roth

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