PMID: 8947481Nov 15, 1996Paper

Impairment by interleukin 1 beta and tumour necrosis factor alpha of the glucagon-induced increase in phosphoenolpyruvate carboxykinase gene expression and gluconeogenesis in cultured rat hepatocytes

The Biochemical Journal
B Christ, A Nath

Abstract

The influence of the inflammatory mediators interleukin 1 beta (IL1 beta) and tumour necrosis factor alpha (TNF alpha) on the glucagon-induced expression of phosphoenolpyruvate carboxykinase (PCK) and on glucose formation via gluconeogenesis was investigated in cultured rat hepatocytes. Gene expression was monitored by determination of mRNA levels and of enzyme activity. Glucose formation was estimated with newly synthesized radioactive glucose derived from a radiolabelled lactate precursor. Glucagon (0.1 or 1 nM) induced PCK mRNA transiently to a maximum 2 h after its application. In the presence of recombinant human (rh) IL1 beta or rhTNF alpha the increase in PCK mRNA levels was totally inhibited at 0.1 nM glucagon, whereas at 1 nM glucagon the maximal increase was inhibited by only 25%. Glucagon (0.1 or 1 nM) induced PCK activity to a maximum after 4 h (4-fold and 6-fold over prestimulatory activity respectively). In the presence of rhIL1 beta or rhTNF alpha the maximal increase was inhibited by approx. 50%. Addition of rhIL1 beta or rhTNF alpha 2 h after glucagon, at the maximal glucagon-induced PCK mRNA levels, accelerated the decay of PCK mRNA. Glucagon (1 or 10 nM) [corrected] increased glucose formation from lactate by...Continue Reading

Citations

Sep 7, 2007·Histochemistry and Cell Biology·Bianca Lienenlüke, Bruno Christ
May 7, 2008·Biochemistry. Biokhimii︠a︡·I F Usynin, L E Panin
Mar 4, 2005·The International Journal of Biochemistry & Cell Biology·Josep M ArgilésFrancisco J López-Soriano
Aug 15, 2017·Journal of Diabetes Investigation·Hiroshi TanakaMasaya Hosokawa
Jul 16, 2002·The Journal of Biological Chemistry·Mary E Waltner-LawDaryl K Granner
Jul 17, 2004·Shock·Brian G HarbrechtBaochun Zhang
Feb 3, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·B ChristA Nath
Aug 3, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Eun Ju BaeSang Geon Kim
Mar 19, 2002·Endocrinology·Chithra N KeembiyehettySolomon S Solomon
May 11, 2012·Journal of Animal Science·C A GiffordC R Krehbiel
Apr 16, 2002·Current Opinion in Clinical Nutrition and Metabolic Care·Minnie Holmes-McNary
Jul 27, 2000·The Journal of Biological Chemistry·M Waltner-LawD K Granner
May 8, 1999·Medicinal Research Reviews·J M Argilés, F J López-Soriano
May 19, 2004·The International Journal of Biochemistry & Cell Biology·Stephanie T YerkovichGeorge C T Yeoh

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