Impairment of dentate gyrus neuronal progenitor cell differentiation in a mouse model of temporal lobe epilepsy

Experimental Neurology
Debora A LedergerberJason E Kralic

Abstract

Unilateral intrahippocampal injection of kainic acid (KA) in adult mice induces an epileptic focus replicating major histopathological features of temporal lobe epilepsy (TLE). In this model, neurogenesis is impaired in the lesioned dentate gyrus, although cell proliferation transiently is increased bilaterally in the subgranular zone (SGZ). To investigate further the relationship between epileptogenesis and neurogenesis, we compared the differentiation of cells born shortly before and after KA injection. Immunohistochemical staining for doublecortin and PSA-NCAM, two markers of young neurons, revealed a rapid downregulation of both markers ipsilaterally, whereas they were increased transiently on the contralateral side. To determine whether KA treatment directly affects neural progenitors in the SGZ, dividing cells were prelabeled with 5'-bromo-2'deoxyuridine (BrdU) treatment before unilateral injection of KA. Double staining with the proliferation marker PCNA showed that prelabeled BrdU cells survived KA exposure and proliferated bilaterally. Unexpectedly, the neuronal differentiation of these cells, as assessed after 2 weeks with doublecortin and NeuN triple-staining, occurred to the same extent as on the contralateral side....Continue Reading

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Citations

Apr 23, 2014·PloS One·Rebecca L KowNeil M Nathanson
Sep 4, 2013·Molecular Neurobiology·Longze ShaQi Xu
Feb 24, 2012·Pharmacological Reports : PR·Marta Andres-MachJarogniew J Łuszczki
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Jul 6, 2010·The European Journal of Neuroscience·Venceslas Duveau, Jean-Marc Fritschy
Mar 17, 2015·The Journal of Comparative Neurology·Francine DeprezJean-Marc Fritschy
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Dec 23, 2017·Scientific Reports·Bethany E HosfordSteve C Danzer
Jun 5, 2018·The European Journal of Neuroscience·Tilo GschwindJean-Marc Fritschy
Oct 20, 2020·Frontiers in Neuroscience·Daniela M S MouraMarcos R Costa

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