Impairment of endothelial nitric oxide synthase causes abnormal fat and glycogen deposition in liver

Biochimica Et Biophysica Acta
Lorenz SchildGerburg Keilhoff

Abstract

Nitric oxide (NO) affects fatty acid synthesis and biogenesis of fatty acid consuming mitochondria. However, whether NO generated by the endothelial NO synthase isoform (eNOS) has significant impact on the synthesis and deposition of fat in liver remained unclear. We analyzed the quantity and distribution of mitochondria and fat in liver of wild-type (WT) mice and mice lacking eNOS (eNOS-KO). The livers of eNOS-KO mice contained tenfold more fat close (zone 1) and twenty fold more distal (zone 3) to the artery. The fat was deposited as droplets co-localized with mitochondria. Additionally, the livers of eNOS-KO mice contained 1.5-fold more homogenously distributed glycogen. No difference in the quantity of mitochondria was found between liver homogenates of eNOS-KO mice and WT animals. Mitochondria from liver homogenates of eNOS-KO mice exhibited a higher ratio of citrate synthase (CS) and NADH-cytochrome c oxidoreductase (KI+III) activity. We conclude that lack of eNOS-derived NO stimulates citrate- and lipid synthesis in liver thus contributing to the development of overweight. In support of this view, more visceral fat and 70% higher body weight was determined in one year old eNOS-KO mice in comparison to WT animals.

References

May 1, 1994·The Biochemical Journal·R A HortonM A Titheradge
Mar 1, 1997·Methods : a Companion to Methods in Enzymology·J C Drapier
Jan 1, 1997·Developmental Neuroscience·J E BrenmanD S Bredt
Apr 18, 1998·The Biochemical Journal·F SprangersA J Meijer
Sep 16, 2000·Biochemical Society Symposium·G C Brown, V Borutaite
Feb 8, 2003·Science·Enzo NisoliMichele O Carruba
Nov 1, 1960·Stain Technology·K C RICHARDSONE H FINKE
Jul 28, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Iman MomkenVladimir Veksler
Oct 2, 2004·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Enzo Nisoli, Michele O Carruba
Dec 30, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Michael M KimJoseph A Califano
Jul 19, 2005·Proceedings of the National Academy of Sciences of the United States of America·Tsuyoshi MorishitaNobuyuki Yanagihara
Mar 10, 2006·The Journal of Nutritional Biochemistry·Wenjuan Shi JobgenGuoyao Wu

❮ Previous
Next ❯

Citations

Sep 15, 2010·Proceedings of the National Academy of Sciences of the United States of America·Paschalis-Thomas DouliasHarry Ischiropoulos
Mar 3, 2012·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·C MüllerL Schild
Aug 1, 2015·British Journal of Pharmacology·Frédéric CapelBéatrice Morio
Mar 1, 2016·Nitric Oxide : Biology and Chemistry·Xiaoyu LiuYingjie Chen
Sep 1, 2015·Pharmacological Reports : PR·Edyta MaslakStefan Chlopicki
Jul 24, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Xiaoyu LiuYingjie Chen
Jun 1, 2014·Free Radical Biology & Medicine·Brian E Sansbury, Bradford G Hill
Feb 17, 2009·Developmental Cell·Peter FraislPeter Carmeliet
Jun 22, 2014·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Bohdana M RovenkoVolodymyr I Lushchak
Jan 26, 2013·Diabetes·Sanshiro TateyaFrancis Kim
Jan 3, 2013·Science Signaling·Paschalis-Thomas DouliasHarry Ischiropoulos
Feb 28, 2018·Clinical and Experimental Pharmacology & Physiology·Aneta StachowiczRyszard Korbut
Oct 25, 2017·BioFactors·Khan Mohammad ImranYong-Sik Kim
Jan 31, 2020·Arteriosclerosis, Thrombosis, and Vascular Biology·Brian P DickFrancis Kim
Dec 29, 2018·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Jiyuan PiaoHyun Sook Hong
Aug 24, 2018·JCI Insight·Margarita TenopoulouHarry Ischiropoulos
Oct 13, 2020·F1000Research·Margarita Tenopoulou, Paschalis-Thomas Doulias
Feb 24, 2019·Journal of Hepatology·Adel Hammoutene, Pierre-Emmanuel Rautou
Oct 20, 2021·Experimental Dermatology·Mao-Qiang ManPeter M Elias

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
Lorenz SchildGerburg Keilhoff
Nature Reviews. Cancer
Dai FukumuraRakesh K Jain
Cardiovascular Research
Guy C Brown, Vilmante Borutaite
© 2021 Meta ULC. All rights reserved