Impairment of H+-K+-ATPase-dependent proton transport and inhibition of gastric acid secretion by ethanol

American Journal of Physiology. Gastrointestinal and Liver Physiology
J C Del ValleJ E Felíu

Abstract

Ethanol (1-20% vol/vol) caused a dose-dependent reduction in the basal rate of acid formation in isolated rabbit gastric glands with a calculated EC(50) value of 4.5 +/- 0.2%. Ethanol also reduced ATP levels in isolated gastric glands and in cultured parietal cells (EC(50): 8.8 +/- 0.4% and 8.5 +/- 0.2%, respectively) and decreased both basal and forskolin-stimulated cAMP levels. In studies carried out in gastric gland microsomes, ethanol inhibited the hydrolytic activity of H+-K+-ATPase(EC(50): 8.5 +/- 0.6%), increased passive proton permeability (EC(50): 7.9%), and reduced H+-K+-ATPase-dependent proton transport (EC(50): 3%). Our results show that the inhibition of gastric acid secretion observed at low concentrations of ethanol (< or =5%) is mainly caused by the specific impairment of H+-K+-ATPase-dependent proton transport across cell membranes rather than inhibition of the hydrolytic activity of H+-K+-ATPase, reduction in the cellular content of ATP, or increase in the passive permeability of membranes to protons, although these changes, in combination, must be relevant at concentrations of ethanol > or =7%.

References

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Citations

Jul 2, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Michael K JonesTakuji Shirasawa
Oct 13, 2006·Current Opinion in Gastroenterology·Mitchell L Schubert
Dec 25, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·María SalvatellaJ E Felíu

Related Concepts

Cyclic AMP, (R)-Isomer
Striadyne
Ethanol
Metazoa
Stains
Gastric Acid
Structure of Pyloric Gland
Intracellular Membranes
Microsomes, Liver
Parietal Cells, Gastric

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