Impairment of NADH dehydrogenase and regulation of anaerobic metabolism by the small RNA RyhB and NadE for improved biohydrogen production in Enterobacter aerogenes

Biotechnology for Biofuels
Yan WuYuan Lu

Abstract

Enterobacter aerogenes is a facultative anaerobe and is one of the most widely studied bacterial strains because of its ability to use a variety of substrates, to produce hydrogen at a high rate, and its high growth rate during dark fermentation. However, the rate of hydrogen production has not been optimized. In this present study, three strategies to improve hydrogen production in E. aerogenes, namely the disruption of nuoCDE, overexpression of the small RNA RyhB and of NadE to regulate global anaerobic metabolism, and the redistribution of metabolic flux. The goal of this study was to clarify the effect of nuoCDE, RyhB, and NadE on hydrogen production and how the perturbation of NADH influences the yield of hydrogen gas from E. aerogenes. NADH dehydrogenase activity was impaired by knocking out nuoCD or nuoCDE in E. aerogenes IAM1183 using the CRISPR-Cas9 system to explore the consequent effect on hydrogen production. The hydrogen yields from IAM1183-CD(∆nuoC/∆nuoD) and IAM1183-CDE (∆nuoC/∆nuoD/∆nuoE) increased, respectively, by 24.5 and 45.6% in batch culture (100 mL serum bottles). The hydrogen produced via the NADH pathway increased significantly in IAM1183-CDE, suggesting that nuoE plays an important role in regulating N...Continue Reading

References

Sep 17, 2003·Genes & Development·Eric MasséSusan Gottesman
Feb 9, 2005·The Journal of Biological Chemistry·Ralf JauchMarkus C Wahl
Oct 4, 2005·Journal of Bacteriology·Eric MasséSusan Gottesman
Apr 14, 2009·Nature Methods·Daniel G GibsonHamilton O Smith
May 28, 2010·Nature·Rouslan G EfremovLeonid A Sazanov
Nov 16, 2011·Biotechnology Letters·Zhen KangQingsheng Qi
Oct 26, 2013·Nature Protocols·F Ann RanFeng Zhang
Aug 27, 2014·Proceedings of the National Academy of Sciences of the United States of America·Jennifer S McDowallFrank Sargent
Sep 27, 2014·Bioresource Technology·Fabiana PassosIvet Ferrer
Feb 1, 2015·Applied and Environmental Microbiology·Yu JiangSheng Yang
Jan 21, 2016·Nature·Matthias P Machner, Gisela Storz
Apr 8, 2017·Journal of Biotechnology·Nan XuLiming Liu

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Citations

Jun 6, 2019·Journal of Industrial Microbiology & Biotechnology·Justin M VentoChase L Beisel
Sep 12, 2018·Biotechnology for Biofuels·Xianbo SuDaping Xia
May 1, 2021·Microorganisms·Ruben D Arroyo-OlarteEdgar Morales-Ríos
Jun 15, 2021·EcoSal Plus·Nicholas Backes, Gregory J Phillips

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Methods Mentioned

BETA
PCR
gene knockout

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