Impairment of the developmental potential of frozen-thawed human zygotes obtained after intracytoplasmic sperm injection

Fertility and Sterility
E MacasP J Keller

Abstract

To evaluate the effects of cryopreservation on the survival, cleavage, and morphology of embryos and on the implantation and embryonic loss rates of human zygotes obtained after ICSI compared with frozen-thawed zygotes obtained after traditional IVF. A further objective was to evaluate the same parameters in nonfrozen sibling ICSI and IVF zygotes and to compare them with corresponding frozen-thawed zygotes. Open, retrospective, comparative study. University-associated assisted reproductive program. Couples with severe male factor infertility and couples undergoing IVF during the same period. A cohort of 408 ICSI zygotes and 299 IVF zygotes was frozen in 1,2 propanediol and sucrose using a slow-freezing protocol. Both groups of zygotes were frozen at approximately the same time after microassisted or conventional insemination. One hundred and eighty-seven ICSI and 110 IVF frozen zygotes were rapidly thawed during 44 ICSI cycles and 24 IVF cycles. Zygotes that appeared to have survived were cultured for 24 hours, and most of these embryos that were morphologically normal were transferred into patients. Survival rate (morphologically intact after thawing), cleavage rate and morphology of embryos, implantation rate, and the inciden...Continue Reading

Citations

Aug 26, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J Tesarik, C Mendoza
Mar 26, 2004·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Y Menezo
Oct 16, 2003·Placenta·E M KolibianakisP Devroey
Jan 13, 2001·Molecular and Cellular Endocrinology·E Van den AbbeelA Van Steirteghem
Jan 13, 2001·Molecular and Cellular Endocrinology·N Nikolettos, S Al-Hasani
Jan 21, 2009·Reproduction, Fertility, and Development·A Dinnyes, T L Nedambale
Nov 5, 1999·Human Reproduction·H JorisA Van Steirteghem
Jun 27, 2012·Journal of Assisted Reproduction and Genetics·Wei FanZhun Xiao
Feb 18, 2011·Reproductive Biomedicine Online·Ervin MacasBruno Imthurn
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Dec 5, 2003·Reproductive Biomedicine Online·J GerrisM Vercruyssen
Jul 6, 2010·Fertility and Sterility·Marie PradesCatherine Poirot

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