IMPDH2 promotes colorectal cancer progression through activation of the PI3K/AKT/mTOR and PI3K/AKT/FOXO1 signaling pathways

Journal of Experimental & Clinical Cancer Research : CR
Shiyu DuanJun Zhou

Abstract

Inosine 5'-monophosphate dehydrogenase type II (IMPDH2) was originally identified as an oncogene in several human cancers. However, the clinical significance and biological role of IMPDH2 remain poorly understood in colorectal cancer (CRC). Quantitative real-time polymerase chain reaction (qPCR), western blotting analysis, the Cancer Genome Atlas (TCGA) data mining and immunohistochemistry were employed to examine IMPDH2 expression in CRC cell lines and tissues. A series of in-vivo and in-vitro assays were performed to demonstrate the function of IMPDH2 and its possible mechanisms in CRC. IMPDH2 was upregulated in CRC cells and tissues at both mRNA and protein level. High IMPDH2 expression was closely associated with T stage, lymph node state, distant metastasis, lymphovascular invasion and clinical stage, and significantly correlated with poor survival of CRC patients. Further study revealed that overexpression of IMPDH2 significantly promoted the proliferation, invasion, migration and epithelial-mesenchymal transition (EMT) of CRC cells in vitro and accelerated xenograft tumour growth in nude mice. On the contrary, knockdown of IMPDH2 achieved the opposite effect. Gene set enrichment analysis (GSEA) showed that the gene set r...Continue Reading

References

Aug 5, 2020·Technology in Cancer Research & Treatment·Yu-Shui MaDa Fu
Apr 10, 2019·Journal of Cellular Biochemistry·Sheng YangGeyu Liang
Jul 24, 2020·Journal of Biochemistry·Satoshi Kofuji, Atsuo T Sasaki
Oct 22, 2020·Cell Biology International·Nuan WangChengxia Liu

Citations

Mar 31, 1999·Proceedings of the National Academy of Sciences of the United States of America·T D ColbyB M Goldstein
Aug 22, 2002·Nature Reviews. Cancer·Jean Paul Thiery
Nov 19, 2004·Nature·Joan Massagué
Sep 15, 2005·The Journal of Cell Biology·Philippe R J BoisGerard C Grosveld
May 8, 2007·Medicinal Research Reviews·Qingning Shu, Vasu Nair
Jul 31, 2007·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Joerg FellenbergAnita Zahlten-Hinguranage
Nov 8, 2007·Proceedings of the National Academy of Sciences of the United States of America·Wendy RoebKaren C Arden
Apr 15, 2008·Trends in Molecular Medicine·Nancy Gavert, Avri Ben-Ze'ev
Jun 2, 2009·Chemical Reviews·Lizbeth Hedstrom
May 31, 2011·Cellular Signalling·Ingeborg HersJeremy M Tavaré
Feb 4, 2012·Biochemical and Biophysical Research Communications·Hwa-Ryeon KimHong-Duk Youn
Jan 17, 2013·Journal of Cancer Research and Clinical Oncology·Berrin TuncaTurkkan Evrensel
Sep 12, 2014·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jun-ichi KawadaHiroshi Kimura
Feb 6, 2015·CA: a Cancer Journal for Clinicians·Lindsey A TorreAhmedin Jemal
Jan 12, 2017·Cell Reports·Khian Hong PuaGregory L Verdine
Mar 29, 2018·Journal of Experimental & Clinical Cancer Research : CR·Yen-Hsiu YehTsai-Kun Li

Related Concepts

Biological Markers
Real-Time Polymerase Chain Reaction
Study
In Vivo
Biochemical Pathway
FOXO1 gene
IMPDH2
The Cancer Genome Atlas
Skeletal Muscle Hypertrophy Pathway
Transfection

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