PMID: 8603084Mar 13, 1996Paper

Impedance analysis of supported lipid bilayer membranes: a scrutiny of different preparation techniques

Biochimica Et Biophysica Acta
C SteinemH J Galla

Abstract

One topic of this study is the comparison of different preparation techniques to build up solid supported lipid bilayers onto gold substrates. The deposited lipid bilayers were investigated by a.c. impedance spectroscopy. Three different strategies were applied: (1) The gold surface was initially covered with a chemisorbed monolayer of octadecanethiol or 1,2-dimyristoyl-sn-glycero-3-phosphothioethanol (DMPTE). The second monolayer consisting of phospholipids was then deposited onto this hydrophobic surface by (i) the Langmuir-Schaefer-technique, (ii) from lipid solution in n-decane/isobutanol, (iii) by the lipid/detergent dilution technique or (iv) by fusion of vesicles. (2) Charged molecules carrying thiol-anchors for attachment to the gold surface by chemisorption were used. Negatively charged surfaces of 3-mercaptopropionic acid were found to be excellent substrates that allow the attachment of planar lipid bilayers by applying positively charged dimethyldioctadecylammoniumbromide (DODAB) vesicles or negatively charged 1,2-dipalmitoyl-sn-glycero-3-phosphoglycerol vesicles in the presence of chelating Ca2+-ions. If positively charged first monolayers of mercaptoethylammoniumhydrochloride were used we were able to attach mixed...Continue Reading

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Citations

Apr 7, 2006·Analytical and Bioanalytical Chemistry·Andreas Janshoff, Claudia Steinem
Nov 12, 2005·European Biophysics Journal : EBJ·Monika NaumowiczZbigniew Figaszewski
Jul 10, 1996·Journal of Biochemical and Biophysical Methods·J WegenerH J Galla
Apr 28, 2004·Bioelectrochemistry·M R MoncelliS M Schiller
Dec 8, 2011·Analytical Chemistry·Irep Gözen, Aldo Jesorka
Sep 13, 2012·Analytical Chemistry·Nathan J WittenbergSang-Hyun Oh
Apr 9, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Roger MichelDavid G Castner
Jan 16, 2007·Journal of Biomolecular Screening·Viera LukacovaStefan Balaz
Sep 9, 2004·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Yuka MatsunoMasao Sugawara
Mar 24, 2011·Journal of Oleo Science·Takakuni OguchiMasahiko Abe
Sep 1, 2008·Biointerphases·Magnus P JonssonFredrik Höök
Apr 23, 2010·Biointerphases·Andreas O HohnerJoachim O Rädler
May 21, 2014·Analytical and Bioanalytical Chemistry·Daniel F KienleTonya L Kuhl
Apr 14, 2010·International Journal of Molecular Sciences·Radek Machán, Martin Hof
Jun 22, 2010·Current Opinion in Biotechnology·Sheereen MajdMichael Mayer
Feb 26, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Julius L ZielenieckiIngo Köper
Jul 25, 2007·Journal of Biomedical Materials Research. Part a·D ThidJ Gold
Nov 27, 2007·Journal of Peptide Science : an Official Publication of the European Peptide Society·Minako KaiShunsaku Kimura
Aug 16, 2008·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Robert F RoskampIngo Köper
Mar 2, 2010·Biochimica Et Biophysica Acta·Radek Machán, Martin Hof
Jul 9, 2016·The Journal of Chemical Physics·Hsing-Lun WuYu-Jane Sheng
Apr 3, 2007·Biophysical Journal·Midhat H Abdulreda, Vincent T Moy
Sep 11, 2012·Biochimica Et Biophysica Acta·Camilla A CarvalhoAna M Carmona-Ribeiro
Aug 9, 2005·Biophysical Journal·Jan K Rainey, Brian D Sykes
Sep 4, 2004·Biophysical Journal·Fernanda F RossettiMarcus Textor
Feb 2, 2008·Biochimica Et Biophysica Acta·Simon FaissClaudia Steinem

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