Impedance monitoring of bacterial activity

Journal of Medical Microbiology
A Ur, D F Brown

Abstract

Bacterial activity and growth were monitored by following the changes of electrical impedance of cultures in liquid media. The signal is expressed automatically as a curve similar to grwoth curves produced by other methods. The technique offers a new, rapid and sensitive means of detecting active micro-organisms and is potentially the basis of rapid automated systems in this field. The impedance changes indicate that the micro-organisms metabolise substrates of low conductivity into products of high conductivity and that the changes are due to the activity of the micro-organisms rather than increase in their numbers. The activity of strains of Escherichia coli, Klebsiella aerogenes, Pseudo-monas aeruginosa, Staphyloccus aureus, and Streptococcus faecalis was detected within 2 h with inocula of 10-3 minus 10-5 organisms per ml. Different reactions of bacteria in various media suggest that the method may be applied to the rapid identification of micro-organisms. The inhibitory effect of antibiotics on bacteria was demonstrated within 2 h, indicating that the method may be useful for the rapid determination of bacterial sensitivity to antibiotics and the rapid assay of antibiotics in serum. Correlation of response time to initial ...Continue Reading

Citations

Jan 1, 1991·Veterinary Research Communications·J Vaid
Oct 1, 1988·Journal of Biomedical Engineering·C J FeliceB V Gallo
Jan 1, 1985·Biosensors·C M Harris, D B Kell
Mar 1, 1988·Diagnostic Microbiology and Infectious Disease·D Amsterdam
Mar 17, 1999·Journal of Microbiological Methods·C J FeliceM E Valentinuzzi
Aug 1, 1978·The Journal of Hygiene·J M Kramer, R J Gilbert
Mar 1, 1996·The Journal of Applied Bacteriology·P Silley, S Forsythe
Jul 1, 1977·Antimicrobial Agents and Chemotherapy·H J Colvin, J C Sherris
Dec 25, 2007·Biotechnology Advances·Liju Yang, Rashid Bashir
Dec 1, 1992·IEEE Transactions on Bio-medical Engineering·C J FeliceR E Madrid
May 16, 2015·Critical Reviews in Food Science and Nutrition·Shyam Narayan JhaRishi Bhardwaj
Sep 17, 2005·Biotechnology and Bioengineering·Liju YangRashid Bashir
Jan 1, 2012·Biosensors·Malcolm J FarrowPatricia Connolly
Oct 6, 2007·The International Journal of Artificial Organs·D CampocciaC R Arciola
Oct 19, 2019·Analytica Chimica Acta·Sergi Brosel-OliuAndrey Bratov

Related Concepts

Ukapen
Antibiotics
Bacteriological Techniques
Cell Density
Electric Conductivity
Alkalescens-Dispar Group
Hydrogen-Ion Concentration
Kantrex
Klebsiella
Pseudomonas aeruginosa

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.