Impedimetric cell-based biosensor for real-time monitoring of cytopathic effects induced by dengue viruses

Biosensors & Bioelectronics
Ming Soon ChengVincent T Chow

Abstract

We describe an impedimetric cell-based biosensor constructed from poly-l-lysine (PLL)-modified screen-printed carbon electrode for real-time monitoring of dengue virus (DENV) infection of surface-immobilized baby hamster kidney (BHK-21) fibroblast cells. Cytopathic effects (CPE) induced by DENV-2 New Guinea C strain (including degenerative morphological changes, detachment, membrane degradation and death of host cells), were reflected by drastic decrease in impedance signal response detected as early as ~30 hours post-infection (hpi). In contrast, distinct CPE by conventional microscopy was evident only at ~72 hpi at the corresponding multiplicity of infection (MOI) of 10. A parameter that describes the kinetics of cytopathogenesis, CIT50, which refers to the time taken for 50% reduction in impedance signal response, revealed an inverse linear relationship with virus titer and MOI. CIT50 values were also delayed by 31.5h for each order of magnitude decrease in MOI. Therefore, based on the analysis of CIT50, the virus titer of a given sample can be determined from the measured impedance signal response. Furthermore, consistent impedance results were also obtained with clinical isolates of the four DENV serotypes verified by RT-P...Continue Reading

References

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Citations

Dec 21, 2016·Journal of Molecular Recognition : JMR·Martin PeslVladimir Rotrekl
Apr 24, 2021·Journal of Biomedical Research·Mihaela Gheorghiu
Aug 9, 2021·Talanta·Taís Aparecida Reis CordeiroLucas Franco Ferreira

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Datasets Mentioned

BETA
KP119840

Methods Mentioned

BETA
nucleic acid amplification
biosensor
biosensing
DropSens
PCR
biosensors
electrophoresis

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