Impedimetric transduction of swelling in pH-responsive hydrogels

The Analyst
Nicky Mac KennaAoife Morrin

Abstract

A pH-responsive hydrogel composed of an aliphatic diamine cross-linked with polyethylene glycol diglycidyl ether (PEGDGE) using a single, rapid polymerisation step has been used to detect glucose by entrapping glucose oxidase (GOx) within its cationic network. The swelling response of hydrogel disks on exposure to glucose were optimised through variation of factors including the cross-linking density of the network, GOx loading and the addition of catalase. Hydrogel-modified carbon cloth electrodes were also prepared and characterised using voltammetric and impedimetric techniques. Non-faradaic electrochemical impedance spectroscopy (EIS) and gravimetry were both employed to track the swelling response of the gels quantitatively. The clear potential of utilising impedance to transduce hydrogel swelling was demonstrated where a linear decrease in gel resistance (Rgel) corresponding to the swelling response was observed in the range 1 to 100 μM. A dramatic increase in the limit of detection of six orders of magnitude over the gravimetric measurement was achieved (from 0.33 mM to 0.08 μM). This increased sensitivity, coupled with the textile-based electrode substrate approach opens the potential applicability of this system for mo...Continue Reading

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Citations

Mar 30, 2016·Biosensors & Bioelectronics·Hyun Seok SongNatalie Artzi
Oct 5, 2016·Journal of the Mechanical Behavior of Biomedical Materials·A D Drozdov, J deC Christiansen
Nov 15, 2018·Micromachines·Olutosin Charles FawoleMassood Tabib-Azar
Mar 15, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Laure FillaudVincent Noel

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