PMID: 8605729Mar 1, 1996Paper

Implantation of human colorectal carcinoma cells in the liver studied by in vivo fluorescence videomicroscopy

Clinical & Experimental Metastasis
S IshiiJ M Jessup

Abstract

In vivo fluorescence videomicroscopy (IVFM) was used to analyse the behavior of weakly and highly metastatic human colorectal carcinoma (CRC) cells during implantation in the liver. A highly metastatic human CRC cell line, CX-1, and a weakly metastatic line, Clone A, were double-labeled with rhodamine B isothiocyanate-dextran (Rd-Dx) to locate cells and with calcein AM to assess cell metabolic activity in an experimental metastasis model. Double-labeled CRC cells (2.0 x 10(6)) were injected into the spleens of groups of nude mice and the livers observed by IVFM over the next 72 h. CRC cells were implanted within 30 s after injection into either portal venules or the proximal third of hepatic sinusoids. Approximately 0.5% of CRC cells traversed the liver through portal-central venous shunts and implanted in the lung. The number of CX-1 cells in the liver was similar to that of Clone A cells during the first 12 h. However, more CX-1 cells than Clone A cells remained in the liver at 4 h and were in groups of 8-12 cells whereas only a few, single Clone A cells were detected in the liver at 72 h. Not all Clone A cells are committed to die within 4 h of implantation because cells harvested 4 h after hepatic implantation proliferated ...Continue Reading

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Citations

Jun 5, 1999·Cell Biochemistry and Biophysics·R L SchultzeP Thomas
Mar 25, 2009·Cancer Microenvironment : Official Journal of the International Cancer Microenvironment Society·Fernando Vidal-Vanaclocha
May 20, 2003·Journal of Gastroenterology and Hepatology·Tae Sook HwangYoung-Mee Park
Apr 14, 2007·Radiology·Jonathan B KruskalS Nahum Goldberg
Sep 24, 2004·International Journal of Cancer. Journal International Du Cancer·Maarten TimmersFilip Braet
Mar 14, 2002·The American Journal of Pathology·Young K SongYong J Lee
Jan 23, 1999·Japanese Journal of Cancer Research : Gann·T OgoshiS Matsuno
Jun 29, 2004·International Journal of Cancer. Journal International Du Cancer·John Milburn JessupPeter Thomas
Jul 23, 2005·Clinical & Experimental Metastasis·J Milburn JessupL M Laguinge
Jan 30, 2020·Advanced Healthcare Materials·Mark F Coughlin, Roger D Kamm

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