Implantation of Neuropixels probes for chronic recording of neuronal activity in freely behaving mice and rats.

Nature Protocols
Rik J J van DaalSebastian Haesler

Abstract

How dynamic activity in neural circuits gives rise to behavior is a major area of interest in neuroscience. A key experimental approach for addressing this question involves measuring extracellular neuronal activity in awake, behaving animals. Recently developed Neuropixels probes have provided a step change in recording neural activity in large tissue volumes with high spatiotemporal resolution. This protocol describes the chronic implantation of Neuropixels probes in mice and rats using compact and reusable 3D-printed fixtures. The fixtures facilitate stable chronic in vivo recordings in freely behaving rats and mice. They consist of two parts: a covered main body and a skull connector. Single-, dual- and movable-probe fixture variants are available. After completing an experiment, probes are safely recovered for reimplantation by a dedicated retrieval mechanism. Fixture assembly and surgical implantation typically take 4-5 h, and probe retrieval takes ~30 min, followed by 12 h of incubation in probe cleaning agent. The duration of data acquisition depends on the type of behavioral experiment. Since our protocol enables stable, chronic recordings over weeks, it enables longitudinal large-scale single-unit data to be routinely...Continue Reading

References

Dec 20, 2013·Journal of Neurophysiology·Antal BerényiGyörgy Buzsáki
Dec 25, 2013·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Tom HartleyJohn O'Keefe
Feb 9, 2017·Journal of Neural Engineering·Joshua H SiegleJakob Voigts
May 16, 2017·Current Biology : CB·José Esquivelzeta RabellSebastian Haesler
Jun 7, 2017·Scientific Reports·Jinho ChungSebastien Royer
Mar 29, 2018·Science·Julija KrupicJohn O'Keefe
Nov 30, 2019·Nature·Nicholas A SteinmetzKenneth D Harris
Dec 4, 2019·Journal of Neural Engineering·Rik J J van DaalFabian Kloosterman

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Software Mentioned

Formlabs
Python
JRCLUST
SpikeGLX
Ecephys
Vetbond
Kilosort
OpenEphys
Neuropixels
MATLAB

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