Implantation of polymer scaffolds seeded with neural stem cells in a canine spinal cord injury model

Cytotherapy
Byung Gon KimByung-Woo Yoon

Abstract

Combinatorial approaches employing diverse therapeutic modalities are required for clinically relevant repair of injured spinal cord in human patients. Before translation into the clinic, the feasibility and therapeutic potential of such combinatorial strategies in larger animal species need to be examined. The present study tested the feasibility of implanting polymer scaffolds via neural stem cell (NSC) delivery in a canine spinal cord injury (SCI) model. The poly(lactic-co-glycolic acid) (PLGA) scaffolds seeded with human NSC were implanted into hemisected canine spinal cord. The PLGA scaffolds bridged tissue defects and were nicely integrated with residual canine spinal cord tissue. Grafted NSC survived the implantation procedure and showed migratory behavior to residual spinal cord tissue. Ectopic expression of a therapeutic neurotrophin-3 gene was also possible in NSC seeded within the PLGA scaffolds. Our description of a canine SCI model would be a valuable resource for pre-clinical trials of combinatorial strategies in larger animal models.

References

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Citations

Aug 20, 2014·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Chen ZhangLin Shen
Aug 27, 2014·Stem Cell Research & Therapy·Bikesh ShresthaLi Yao
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Apr 10, 2019·Neural Regeneration Research·Shuo LiuBin Wang
Jul 24, 2020·International Journal of Molecular Sciences·Zuzana VikartovskaDasa Cizkova
Jun 2, 2020·Journal of Veterinary Science·Min Hee Kang, Hee Myung Park
Jan 30, 2018·Frontiers in Cellular Neuroscience·Shengwen LiuRadhika Puttagunta
Oct 17, 2020·Frontiers in Veterinary Science·Melissa J LewisUNKNOWN Canine Spinal Cord Injury Consortium (CANSORT-SCI)
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Feb 3, 2019·Acta Biomaterialia·Qingzheng ZhangXuesi Chen
Nov 17, 2020·Frontiers in Veterinary Science·Melissa J LewisUNKNOWN Canine Spinal Cord Injury Consortium (CANSORT-SCI)
Nov 7, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Nazanin GhaneAmirkianoosh Kiani
Jul 10, 2017·ACS Biomaterials Science & Engineering·Bridget A BreenSiobhan S McMahon

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