Implementation and application of a multiplex assay to detect malaria-specific antibodies: a promising tool for assessing malaria transmission in Southeast Asian pre-elimination areas

Malaria Journal
Karen KerkhofLies Durnez

Abstract

Epidemiological surveillance is a key activity in malaria control and elimination in low-transmission and pre-elimination settings. Hence, sensitive tools for estimating malaria force of infection are crucial. Serological markers might provide additional information in estimating force of infection in low-endemic areas along with classical parasite detection methods. Serological markers can be used to estimate recent, past or present malaria exposure, depending on the used markers and their half-life. An assay based on 14 Plasmodium-specific peptides, one peptide specific for Anopheles gambiae saliva protein and five Plasmodium-specific recombinant proteins was developed for the MAGPIX system, assessed for its performance, and applied on blood spots from 2000 individuals collected in the Ratanakiri Province, Cambodia. A significant correlation for the use of 1000 and 2000 beads/antigen/well as well as for the monoplex versus multiplex assay was observed for all antigens (p < 0.05). For the majority of antigens, antigen-coupled beads were stable for at least 2 months. The assay was very reproducible with limited intercoupling, interplate and intraplate variability (mean RSD <15 %). Estimating seroconversion and seroreversion per...Continue Reading

References

Mar 29, 2005·Proceedings of the National Academy of Sciences of the United States of America·C J DrakeleyE M Riley
Apr 27, 2005·Lancet·Brian M GreenwoodGeoffrey A T Targett
Feb 17, 2006·Methods : a Companion to Methods in Enzymology·Mohamed F Elshal, J Philip McCoy
Oct 13, 2006·Clinical and Vaccine Immunology : CVI·Genevieve G FoudaArmead H Johnson
Jul 20, 2007·Clinical and Vaccine Immunology : CVI·John FerbasSteven J Swanson
Nov 9, 2007·Trends in Parasitology·Patrick CorranChris Drakeley
Mar 11, 2008·Journal of Immunological Methods·James E DrummondDanilo R Casimiro
Jul 18, 2008·Clinical and Vaccine Immunology : CVI·M J BinnickerE M Beito
Oct 18, 2008·Clinical and Vaccine Immunology : CVI·Clare WhelanJohn Clarke
May 20, 2009·Molecular and Biochemical Parasitology·Colin J Sutherland
Jun 3, 2009·The Korean Journal of Parasitology·Noppadon TangpukdeeSrivicha Krudsood
Nov 6, 2009·Helicobacter·Angelika MichelMichael Pawlita
Jun 18, 2010·Malaria Journal·Jackie CookChris Drakeley
Dec 15, 2010·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Cara N Henry-HalldinPeter A Zimmerman
Aug 1, 2012·International Journal for Parasitology·Patrick J LammieJeffrey W Priest
Apr 9, 2013·Trends in Parasitology·Anja Scholzen, Robert W Sauerwein
Jul 29, 2015·Proceedings of the National Academy of Sciences of the United States of America·Danica A HelbBryan Greenhouse

❮ Previous
Next ❯

Citations

Dec 15, 2017·Frontiers in Microbiology·Denis A KleymenovArtem P Tkachuk
Dec 6, 2019·Malaria Journal·Eric RogierVenkatachalam Udhayakumar
Apr 4, 2019·Malaria Journal·Patric Stephane EpopaAbdoulaye Diabate

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
enzyme-linked immunosorbent assay
ELISA

Clinical Trials Mentioned

NCT01663831

Software Mentioned

R
R package ‘ segmented
GeneCust
MAGPIX
VFCV

Related Concepts

Related Feeds

Antimalarial Agents (ASM)

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Antimalarial Agents

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.