Implementation and benchmarking of a novel analytical framework to clinically evaluate tumor-specific fluorescent tracers

Nature Communications
Marjory KollerGooitzen M van Dam

Abstract

During the last decade, the emerging field of molecular fluorescence imaging has led to the development of tumor-specific fluorescent tracers and an increase in early-phase clinical trials without having consensus on a standard methodology for evaluating an optical tracer. By combining multiple complementary state-of-the-art clinical optical imaging techniques, we propose a novel analytical framework for the clinical translation and evaluation of tumor-targeted fluorescent tracers for molecular fluorescence imaging which can be used for a range of tumor types and with different optical tracers. Here we report the implementation of this analytical framework and demonstrate the tumor-specific targeting of escalating doses of the near-infrared fluorescent tracer bevacizumab-800CW on a macroscopic and microscopic level. We subsequently demonstrate an 88% increase in the intraoperative detection rate of tumor-involved margins in primary breast cancer patients, indicating the clinical feasibility and support of future studies to evaluate the definitive clinical impact of fluorescence-guided surgery.

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Citations

May 6, 2019·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Naoki NishioEben L Rosenthal
Dec 11, 2019·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Nicholas E WojtynekAaron M Mohs
Apr 23, 2020·Cancers·Fortuné M K ElekonawoMark Rijpkema
Aug 13, 2020·European Journal of Nuclear Medicine and Molecular Imaging·F B AchterbergA L Vahrmeijer
Mar 13, 2020·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Nicholas E Wojtynek, Aaron M Mohs
Jan 12, 2020·Communications Biology·Guillaume NormandSudeep Chandra
Sep 21, 2020·Journal of Biophotonics·Ghayathri BalasundaramMalini Olivo
Oct 21, 2020·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Shrey KapoorNaoki Nishio
Dec 11, 2020·Journal of Biomedical Optics·Servando Hernandez VargasAli Azhdarinia
Nov 14, 2020·European Journal of Surgical Oncology : the Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·Florin-Catalin PopGabriel Liberale
Feb 18, 2021·The Laryngoscope·Yu-Jin LeeEben L Rosenthal
Jul 19, 2020·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Pieter J SteinkampGooitzen M van Dam
Mar 26, 2021·The Lancet Oncology·Lorraine J LauwerendsStijn Keereweer
May 7, 2021·Science Translational Medicine·Steven BlairViktor Gruev
Oct 4, 2020·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Naoki NishioKatheryne E Wilson
Oct 20, 2019·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Steven J de JonghWouter B Nagengast
Jul 20, 2021·Frontiers in Oncology·Servando Hernandez VargasAli Azhdarinia
Sep 1, 2021·European Journal of Nuclear Medicine and Molecular Imaging·Milou E NoltesRudi A J O Dierckx
Oct 12, 2021·European Journal of Nuclear Medicine and Molecular Imaging·H M SchouwS Kruijff
Nov 6, 2021·European Journal of Nuclear Medicine and Molecular Imaging·J VonkMjh Witjes
Sep 9, 2021·Nature Reviews. Clinical Oncology·J Sven D MieogAlexander L Vahrmeijer

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Methods Mentioned

BETA
fluorescence imaging
fluorescence
imaging techniques
fluorescence microscopy
electrophoresis
pregnancy test
biopsy

Clinical Trials Mentioned

NCT02583568
NCT02113202

Software Mentioned

ImageJ
GraphPad Prism
LAS
Fiji
Photoshop Creative Cloud

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