Implementation of a Fully Automated Microbial Cultivation Platform for Strain and Process Screening

Biotechnology Journal
Nils H JanzenDaniela Reinisch

Abstract

Advances in molecular biotechnology have resulted in the generation of numerous potential production strains. Because every strain can be screened under various process conditions, the number of potential cultivations is multiplied. Exploiting this potential without increasing the associated timelines requires a cultivation platform that offers increased throughput and flexibility to perform various bioprocess screening protocols. Currently, there is no commercially available fully automated cultivation platform that can operate multiple microbial fed-batch processes, including at-line sampling, deep freezer off-line sample storage, and complete data handling. To enable scalable high-throughput early-stage microbial bioprocess development, a commercially available microbioreactor system and a laboratory robot are combined to develop a fully automated cultivation platform. By making numerous modifications, as well as supplementation with custom-built hardware and software, fully automated milliliter-scale microbial fed-batch cultivation, sample handling, and data storage are realized. The initial results of cultivations with two different expression systems and three different process conditions are compared using 5 L scale benc...Continue Reading

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Citations

Aug 11, 2020·Engineering in Life Sciences·Joseph NewtonDaniela Reinisch
Jan 11, 2020·Frontiers in Bioengineering and Biotechnology·Nagesh K Tripathi, Ambuj Shrivastava
Jan 16, 2020·Bioengineering·Johanna HausjellOliver Spadiut
Sep 25, 2021·Microbial Cell Factories·Kaushik RajRadhakrishnan Mahadevan

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