Implementation of a model for identifying Essentially Derived Varieties in vegetatively propagated Calluna vulgaris varieties.

BMC Genetics
Thomas BorchertAnnette Hohe

Abstract

Variety protection is of high relevance for the horticultural community and juridical cases have become more frequent in a globalized economy due to essential derivation of varieties. This applies equally to Calluna vulgaris, a vegetatively propagated species from the Ericaceae family that belongs to the top-selling pot plants in Europe. We therefore analyzed the genetic diversity of 74 selected varieties and genotypes of C. vulgaris and 3 of Erica spp. by means of RAPD and iSSR fingerprinting using 168 mono- and polymorphisms. The same data set was utilized to generate a system to reliably identify Essentially Derived Varieties (EDVs) in C. vulgaris, which was adapted from a method suggested for lettuce and barley. This system was developed, validated and used for selected tests of interest in C. vulgaris. As expected following personal communications with breeders, a very small genetic diversity became evident within C. vulgaris when investigated using our molecular methods. Thus, a dendrogram-based assay to detect Essentially Derived Varieties in this species is not suitable, although varieties are propagated vegetatively. In contrast, the system applied in lettuce, which itself applies pairwise comparisons using appropriate...Continue Reading

References

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Citations

May 3, 2019·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Seyed Hossein JamaliLee T Hickey

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Methods Mentioned

BETA
genotyping
PCR

Software Mentioned

Phoretix
Treeview
NTSYSpc
SimQual
PHYLIP
Winboot

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