Abstract
The Project HOPE (High-tech Omics-based Patient Evaluation) for cancer medicine aims to evaluate biological characteristics of each cancer tissue as well as diathesis of each patient in around 1,000 consecutive cases per year, who receive operations at the Shizuoka Cancer Center. Cancer tissues are investigated by whole-exome sequencing for 18,835 genes, focusing on 12,776 in-house cancer hotspots from 483 cancer-associated genes. To confirm cancer-specific genetic changes, we analyzed blood cells to collate with data of cancer tissues, and we reevaluate cancer tissues by comprehensive cancer panel for 409 genes. In order to investigate diathesis of the patients, we evaluate 43,015 hotspots associated with non-cancerous diseases. In terms of gene expression profiling, we analyze cancer-specific alterations for 29,833 genes using tumor and adjacent normal tissues. If and when necessary, we investigate tumor and normal tissues by proteomics and metabolomics. The model experiments using glioblastoma cell lines demonstrated that the method is appropriate for clinical application. The Project HOPE makes it possible to implement individualized medicine and to practice preventive and presymptomatic medicine for cancer patients. Furthe...Continue Reading
References
Jan 1, 1996·Methods in Enzymology·G D SchulerJ A Kans
Oct 19, 2010·Nucleic Acids Research·Simon A ForbesP Andrew Futreal
Dec 1, 2008·Proteomics. Clinical Applications·Shun-Ichiro OguraTohru Mochizuki
Mar 8, 2011·Cell·Douglas Hanahan, Robert A Weinberg
May 7, 2011·Proteomics·Keiichi HatakeyamaTohru Mochizuki
Jan 1, 2013·The Journal of Molecular Diagnostics : JMD·Carol BeadlingChristopher L Corless
Mar 30, 2013·Science·Bert VogelsteinKenneth W Kinzler
Apr 2, 2013·Cell·Levi A Garraway, Eric S Lander
Jun 22, 2013·Genetics in Medicine : Official Journal of the American College of Medical Genetics·Robert C GreenUNKNOWN American College of Medical Genetics and Genomics
Oct 22, 2013·Nature Biotechnology·Garrett M FramptonRoman Yelensky
Nov 6, 2013·Biomedical Research·Kenichi UrakamiMasatoshi Kusuhara
May 3, 2014·PLoS Genetics·Yoshiko MurakamiRami Abou Jamra
Jul 18, 2014·Experimental Hematology & Oncology·Markus RechsteinerMarianne Tinguely
Jul 30, 2014·Proteomics·Keiichi OhshimaTohru Mochizuki
Citations
Jun 27, 2019·Cancer Medicine·Hitoshi HinoKen Yamaguchi
Dec 22, 2019·Cancer Science·Takeshi NagashimaYasuto Akiyama
Mar 19, 2020·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Kenichi NakamuraMasanori Terashima
Sep 24, 2020·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Keiichi FujiyaKen Yamaguchi
Dec 23, 2016·Biomedical Research·Yuji ShimodaKen Yamaguchi
Mar 23, 2017·The Journal of Toxicological Sciences·Sumiko OhnamiKen Yamaguchi
Jul 28, 2020·Human Genetics·Yasue HoriuchiKen Yamaguchi
Aug 23, 2016·Biomedical Research·Ken YamaguchiMasatoshi Kusuhara
Mar 5, 2017·Molecular and Cellular Biochemistry·Shumpei OhnamiKen Yamaguchi
Dec 23, 2016·Biomedical Research·Takeshi NagashimaKen Yamaguchi
May 19, 2018·Oncotarget·Ryo AshidaKen Yamaguchi
Jul 25, 2020·Medicine·Yoshiyuki IidaTetsuro Onitsuka
Feb 26, 2016·Biomedical Research·Kenichi UrakamiKen Yamaguchi
Aug 23, 2016·Biomedical Research·Yasuto AkiyamaKen Yamaguchi
Feb 28, 2017·Biomedical Research·Rie MakuuchiKen Yamaguchi
Aug 18, 2017·Omics : a Journal of Integrative Biology·Theodora KatsilaDimitrios Kardamakis
Apr 30, 2017·The Journal of Dermatology·Yoshio KiyoharaYasuto Akiyama
Aug 7, 2019·Cancer Medicine·Yoshimi KiyozumiKen Yamaguchi
Apr 6, 2017·Scientific Reports·Keiichi OhshimaKen Yamaguchi
Mar 9, 2017·BMC Gastroenterology·Hiroyuki MatsubayashiHiroyuki Ono
May 16, 2017·Human Genome Variation·Akane NaruokaKen Yamaguchi
Oct 14, 2020·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Yoshimi KiyozumiKen Yamaguchi
Sep 4, 2021·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Kenichiro FurukawaKen Yamaguchi
Oct 1, 2021·Human Genome Variation·Masakuni SerizawaKen Yamaguchi
Dec 7, 2021·Medicine·Rei TanakaKen Yamaguchi