Implementation of Isavuconazole in a Fluorescence-Based High-Performance Liquid Chromatography Kit Allowing Simultaneous Detection of All Four Currently Licensed Mold-Active Triazoles

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René JørgensenMaiken Cavling Arendrup

Abstract

Isavuconazole (ISZ) is a newly available broad-spectrum triazole agent recently approved for the treatment of both invasive aspergillosis and mucormycosis. The aim of this study was to develop a simple and reliable method for therapeutic drug monitoring (TDM) of ISZ in human plasma samples. The method involves using a kit from ChromSystems intended for TDM of itraconazole (ITZ), posaconazole (PSZ), and voriconazole (VRZ) in serum/plasma for sample preparation and high-performance liquid chromatography, using fluorescence detection with emission and excitation wavelengths set to 261 and 366 nm, respectively. The assay was linear over the ISZ concentration range of 0.2 to 20.0 mg/liter, using a 0.1-ml sample volume. The inter- and intraday coefficients of variation were all below 3.7%, whereas the accuracies ranged from 95.0 to 106.2% and the mean extraction recovery was 91.9%. In addition, the method worked well using four different Vacutainer types, with six different healthy volunteers and under a number of relevant storage conditions. Finally, the ISZ detection could be seamlessly implemented in the TDM kit for VRZ, PSZ, and ITZ, enabling simultaneous detection of all four triazoles. This method proved to be simple, accurate,...Continue Reading

References

Aug 21, 2013·Antimicrobial Agents and Chemotherapy·Susan J HowardMaiken C Arendrup
Oct 7, 2015·Antimicrobial Agents and Chemotherapy·Maiken Cavling ArendrupJoseph Meletiadis
Feb 7, 2016·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M C ArendrupUNKNOWN Subcommittee on Antifungal Susceptibility Testing (AFST) of the ESCMID European Committee for Antimicrobial Susceptibility T
Jan 29, 2017·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Balthazar ToussaintVincent Jullien

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Citations

Oct 3, 2018·Antimicrobial Agents and Chemotherapy·Xuemei WuM Hong Nguyen
Apr 6, 2019·Therapeutic Drug Monitoring·Federico PeaAnne Santerre Henriksen
Oct 1, 2019·Therapeutic Drug Monitoring·Federico PeaAnne S Henriksen

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