Implementation of liquid chromatography-high resolution mass spectrometry methods for untargeted metabolomic analyses of biological samples: A tutorial

Analytica Chimica Acta
Julian PezzattiSerge Rudaz

Abstract

Untargeted metabolomics is now widely recognized as a useful tool for exploring metabolic changes taking place in biological systems under different conditions. By its nature, this is a highly interdisciplinary field of research, and mastering all of the steps comprised in the pipeline can be a challenging task, especially for those researchers new to the topic. In this tutorial, we aim to provide an overview of the most widely adopted methods of performing LC-HRMS-based untargeted metabolomics of biological samples. A detailed protocol is provided in the Supplementary Information for rapidly implementing a basic screening workflow in a laboratory setting. This tutorial covers experimental design, sample preparation and analysis, signal processing and data treatment, and, finally, data analysis and its biological interpretation. Each section is accompanied by up-to-date literature to guide readers through the preparation and optimization of such a workflow, as well as practical information for avoiding or fixing some of the most frequently encountered pitfalls.

Citations

Mar 19, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Gaole ZhangDe-An Guo
Oct 22, 2020·Metabolites·Gabriel RiquelmeMaría Eugenia Monge
Nov 29, 2020·Analytical and Bioanalytical Chemistry·Hans H Maurer
Nov 21, 2020·Metabolites·Gerjen H TinneveltJeroen J Jansen
Feb 20, 2021·The Analyst·Eulalia OlestiSerge Rudaz
May 1, 2021·International Journal of Molecular Sciences·David PamiesMarie-Gabrielle Zurich
Jul 3, 2021·Cancers·Diren Beyoğlu, Jeffrey R Idle
Jul 25, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Chiara LavarelloGiuliana Cangemi
Jul 21, 2021·Journal of Proteome Research·Alexandra Bourdin-PintuelesSylvie Mavel
Aug 2, 2021·Food Chemistry·Michele UtpottSimone Hickmann Flôres

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