Implication of a regulator of G protein signalling (BbRGS1) in conidiation and conidial thermotolerance of the insect pathogenic fungus Beauveria bassiana

FEMS Microbiology Letters
Weiguo FangM J Bidochka

Abstract

Beauveria bassiana is an insect pathogenic fungus widely used as a biocontrol agent to infect and control insect pests. The conidium initiates pathogenesis and mediates disease transmission, however, little is known about genetic elements that control conidiation. Here, the cloning and characterization of a regulatory G protein-signalling (RGS) gene Bbrgs1 from B. bassiana is reported. Phylogenetic analysis showed that BbRGS1 was orthologous to the RGS protein, FlbA from Aspergillus nidulans. The disruption of Bbrgs1 resulted in significantly reduced conidial production on agar plates or from infected insects. The DeltaBbrgs1 conidia also demonstrated reduced thermotolerance and conidial viability but germinated significantly faster than the wild type. Although hydrophobins have been implicated in conidial thermal sensitivity, the amount of hydrophobin on the conidial surface and the level of transcription of the hydrophobin-like protein gene hl were unaltered. Virulence toward insects was similar to that of the wild-type or reverse complementation transformants, and the mRNA levels of virulence factor genes for chitinase (Bbchit) and subtilisin-like protease (cdep1), were unchanged in DeltaBbrgs1. Characterization of a single ...Continue Reading

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Citations

Jun 10, 2010·Applied Microbiology and Biotechnology·Javier Palma-GuerreroLuis Vicente Lopez-Llorca
Aug 7, 2013·Applied Microbiology and Biotechnology·Yunchuan WangJinkui Yang
Mar 19, 2014·Applied Microbiology and Biotechnology·Yanchun ShaoFusheng Chen
Jun 17, 2014·Applied Microbiology and Biotechnology·Yueqing CaoYuxian Xia
Dec 3, 2015·Journal of Invertebrate Pathology·Claudio A Valero-JiménezJan A L van Kan
May 21, 2013·Journal of Basic Microbiology·Cheila Denise Ottonelli StopigliaMaria Lúcia Scroferneker
Aug 13, 2014·Current Genetics·Almudena Ortiz-Urquiza, Nemat O Keyhani
Mar 2, 2018·The Journal of Microbiology·Qingqing YanYanchun Shao

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