PMID: 18707587Aug 19, 2008Paper

Implication of alpha5beta1 integrin in invasion of drug-resistant MCF-7/ADR breast carcinoma cells: a role for MMP-2 collagenase

Biochemistry. Biokhimii︠a︡
G E MorozevichA E Berman

Abstract

Expression of alpha5beta1 integrin in the drug-resistant MCF-7/ADR breast carcinoma cells was inhibited by treatment of these cells with alpha5-specific siRNA. The decrease of alpha5beta1 expression resulted in a sharp decrease of expression of MMP-2 collagenase and inhibition of invasion activity of these cells in vitro. Similar decrease of invasion was also observed during inhibition of MMP-2 expression by treatment of these cells with MMP-2-specific siRNA. Inhibition of alpha5beta1 expression was also accompanied by significant decrease in cell content of active (phosphorylated) forms of signal protein kinases Akt and Erk1/2. Inhibition of activity of these kinases by treatment of cells with PI-3K/Akt-specific inhibitor LY294002 or Erk-specific inhibitor PD98059 resulted in inhibition of MMP-2 expression and the decrease of invasion in vitro. These data suggest that alpha5beta1 controls invasion ability of these cells by regulating expression of MMP-2, which involves PI-3K and Erk1/2 protein kinase signaling.

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Citations

Jan 30, 2010·Cancer Metastasis Reviews·R Rathinam, Suresh K Alahari
Jan 19, 2010·American Journal of Respiratory Cell and Molecular Biology·Jesse RomanShouWei Han
Nov 26, 2013·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Peeyush N Goel, Rajiv P Gude
Aug 12, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Ali Mohammad AlizadehSadaf Farsinejad
Aug 31, 2013·Biomedit︠s︡inskai︠a︡ khimii︠a︡·A E BermanG E Morozevich
Jul 28, 2013·Asian Pacific Journal of Cancer Prevention : APJCP·Hua-Yi WangLi-Ming Huang

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