Implication of cartilage intermediate layer protein in cartilage destruction in subsets of patients with osteoarthritis and rheumatoid arthritis

Arthritis and Rheumatism
J TsuruhaK Nishioka

Abstract

To investigate whether cartilage intermediate layer protein (CILP), a protein recently cloned from human articular cartilage, is recognized as an autoantigen in patients with osteoarthritis (OA) and rheumatoid arthritis (RA), and whether the immune response against CILP is involved in disease pathogenesis. Recombinant fusion proteins, which contain the first half (C1), second half (C2), or 3 fragments within the C2 region (designated C2F1, C2F2, and C2F3) of the non-porcine nucleotide pyrophosphohydrolase-homologous region of CILP, were prepared using Escherichia coli. Autoantibodies to these proteins in serum samples from patients with OA or RA and from age-matched healthy individuals were detected by enzyme-linked immunosorbent assay and Western blotting. In addition, mice were immunized with a mixture of the C1 and C2 fusion proteins to assess the arthrogenicity of CILP. Production of antibodies to the C2 region was detected in 10.5% (11 of 105) of the tested OA patients and in 8.0% (7 of 88) of the tested RA patients, although antibodies to the C1 region were rarely detected in either patient group. All C2F1, C2F2, and C2F3 fragments were found to carry autoepitopes. The C2F2 fusion protein was recognized most frequently in...Continue Reading

References

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Citations

Sep 29, 2011·Molecular & Cellular Proteomics : MCP·Stefan LehrJuergen Eckel
May 1, 2002·Current Opinion in Rheumatology·Ikuko Masuda, Jun Hirose
Aug 18, 2004·Current Opinion in Rheumatology·Tomohiro KatoKusuki Nishioka
Jan 8, 2014·Clinical Proteomics·Lavanya BalakrishnanAkhilesh Pandey
Nov 12, 2005·Clinical Anatomy : Official Journal of the American Association of Clinical Anatomists & the British Association of Clinical Anatomists·Riaz A Agha, Barbara Webb
Jan 18, 2014·Immunology·Yanyan GuoYuzhang Wu
Jan 31, 2013·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Abdul Haseeb, Tariq M Haqqi
Mar 13, 2003·Arthritis and Rheumatism·Guo-Hua YuanKusuki Nishioka
Oct 12, 2018·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Shahrzad HosseininiaLeif E Dahlberg
Feb 1, 2007·Arthritis and Rheumatism·Lazaros I Sakkas, Chris D Platsoucas
Feb 1, 2008·Proteomics. Clinical Applications·Richard Wilson, John F Bateman
Jan 19, 2016·Current Opinion in Rheumatology·Karine CheminVivianne Malmström
Feb 24, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yang XiangTomohiro Kato
Dec 22, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Reng-Rong DaYiping Zhang
Jan 24, 2020·Scientific Reports·Siva Sai Krishna VenigallaVani Janakiraman

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