Implication of microRNA regulation in para-phenylenediamine-induced cell death and senescence in normal human hair dermal papilla cells

Molecular Medicine Reports
Ok-Kyu LeeSeunghee Bae

Abstract

Para-phenylenediamine (PPD) is a major component of hair coloring and black henna products. Although it has been largely demonstrated that PPD induces allergic reactions and increases the risk of tumors in the kidney, liver, thyroid gland and urinary bladder, the effect on dermal papilla cells remains to be elucidated. Therefore, the current study evaluated the effects of PPD on growth, cell death and senescence using cell-based assays and microRNA (miRNA) microarray in normal human hair dermal papilla cells (nHHDPCs). Cell viability and cell cycle analyses demonstrated that PPD exhibited a significant cytotoxic effect on nHHDPCs through inducing cell death and G2 phase cell cycle arrest in a dose-dependent manner. It was additionally observed that treatment of nHHDPCs with PPD induced cellular senescence by promoting cellular oxidative stress. In addition, the results of the current study indicated that these PPD-mediated effects were involved in the alteration of miRNA expression profiles. Treatment of nHHDPCs with PPD altered the expression levels of 74 miRNAs by ≥ 2-fold (16 upregulated and 58 downregulated miRNAs). Further bioinformatics analysis determined that these identified miRNA target genes were likely to be involve...Continue Reading

References

Dec 1, 1986·Carcinogenesis·W RojanapoM Tanyakaset
Nov 20, 2002·Pediatric Dermatology·Danielle MarcouxDenis Sasseville
Jul 19, 2003·Science·James C Carrington, Victor Ambros
Jul 23, 2005·IUBMB Life·Renata Colavitti, Toren Finkel
Oct 1, 2005·Proceedings of the National Academy of Sciences of the United States of America·Gerard M J BeaudoinCatherine C Thompson
Feb 14, 2006·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Ssu-Ching ChenSoi-Moi Chye
Nov 17, 2006·The Journal of Pharmacology and Experimental Therapeutics·Eve M CoulterDean J Naisbitt
Dec 14, 2007·Proceedings of the National Academy of Sciences of the United States of America·Daniel Y LeeBurton B Yang
Feb 17, 2010·Toxicology in Vitro : an International Journal Published in Association with BIBRA·S C ChenS M Chye
Jun 5, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Andrei N MardaryevNatalia V Botchkareva
Oct 6, 2010·Advanced Drug Delivery Reviews·Jia-Ling Teo, Michael Kahn
Jul 12, 2011·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Ya-Chun HuangChee-Yin Chai
Jul 12, 2011·Trends in Biochemical Sciences·Guohui WanXiongbin Lu
Dec 21, 2011·Cutaneous and Ocular Toxicology·Gulsen Tukenmez DemirciAsli Kucukunal
Mar 1, 2012·ISRN Dermatology·Waka IshidaTadamichi Shimizu
Nov 16, 2013·Cell Death & Disease·I AmelioE Candi
Feb 26, 2014·The Journal of Allergy and Clinical Immunology. in Practice·Ahmet Zulfikar AkelmaEmin Mete

❮ Previous
Next ❯

Methods Mentioned

BETA
Assay
Feature Extraction
flow cytometry

Software Mentioned

GeneSpring GX
Feature Extraction
DNA Intelligent Analysis ( DIANA ) microT - CDS
DAVID

Related Concepts

Related Feeds

Cancer Epigenetics and Senescence (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may be involved in regulating senescence in cancer cells. This feed captures the latest research on cancer epigenetics and senescence.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.