PMID: 9428015Jan 1, 1997Paper

Implication of pneumolysin as a virulence factor in Streptococcus pneumoniae endophthalmitis

Retina
E W NgDonald J D'Amico

Abstract

To determine if pneumolysin, a multifunctional cytotoxin produced by Streptococcus pneumoniae, may be a virulence determinant in the pathogenesis of pneumococcal endophthalmitis. Lewis rats (n = 20) were injected intravitreally with purified recombinant pneumolysin at the following doses; 3.9 hemolytic units (HU), 39 HU, 390 HU, 3.9 x 10(3) HU, and 3.9 x 10(4) HU. After 24 hours, eyes were examined clinically and enucleated for histopathologic examination to elucidate the dose-response relationship. To determine the temporal progression of the disease model, a second group of rats (n = 8) were injected intravitreally with 390 HU of pneumolysin. At 6 and 48 hours, eyes were examined clinically and enucleated for histopathology. Eyes injected with pneumolysin demonstrated increasing anterior and posterior segment inflammation in response to increasing doses of administered toxin. The onset of inflammation and tissue damage occurred rapidly, and was maximal at 24 to 48 hours. The clinical and histopathologic changes observed mimicked those of S. pneumoniae endophthalmitis. Histopathologic analysis demonstrated rapid onset of iridocyclitis and vitritis with polymorphonuclear leukocyte influx, inner retinal necrosis, and retinal det...Continue Reading

Citations

Nov 3, 2010·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Melissa E SandersMary E Marquart
Oct 5, 2011·Retina·Pierre-Loïc CornutUNKNOWN French Institutional Endophthalmitis Study Group
Sep 14, 2002·Infection and Immunity·Michelle C CalleganDidier Lereclus
Feb 14, 2018·International Journal of Molecular Sciences·Seoh Wei TehSuresh Kumar Subbiah
Sep 16, 2003·Retina·David J WeissgoldMark Iverson
Feb 9, 2012·Acta Ophthalmologica·Pierre-Loïc CornutUNKNOWN French Institutional Endophthalmitis Study (FRIENDS) Group

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