Implication of sphingomyelin/ceramide molar ratio on the biological activity of sphingomyelinase.

Biophysical Journal
Beate BoulgaropoulosGeorg Pabst

Abstract

Sphingolipid signaling plays an important, yet not fully understood, role in diverse aspects of cellular life. Sphingomyelinase is a major enzyme in these signaling pathways, catalyzing hydrolysis of sphingomyelin to ceramide and phosphocholine. To address the related membrane dynamical structural changes and their feedback to enzyme activity, we have studied the effect of enzymatically generated ceramide in situ on the properties of a well-defined lipid model system. We found a gel-phase formation that was about four times faster than ceramide generation due to ceramide-sphingomyelin pairing. The gel-phase formation slowed down when the ceramide molar ratios exceeded those of sphingomyelin and stopped just at the solubility limit of ceramide, due to unfavorable pairwise interactions of ceramide with itself and with monounsaturated phosphatidylcholine. A remarkable correlation to in vitro experiments suggests a regulation of sphingomyelinase activity based on the sphingomyelin/ceramide molar ratio.

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Citations

Jun 5, 2012·European Biophysics Journal : EBJ·G PabstK Lohner
Nov 3, 2011·The Journal of Clinical Investigation·Benjamin T Bikman, Scott A Summers
Feb 12, 2014·Progress in Lipid Research·Bruno M CastroLiana C Silva
Apr 17, 2012·Progress in Lipid Research·Félix M GoñiAlicia Alonso
May 5, 2011·Biophysical Journal·Beate BoulgaropoulosGeorg Pabst
Jul 25, 2012·Biophysical Journal·Beate BoulgaropoulosGeorg Pabst
Jul 7, 2016·American Journal of Physiology. Endocrinology and Metabolism·Hanin AburasaynJohn R Ussher
Oct 4, 2018·The Journal of Nutrition·J Eduardo RicoJoseph W McFadden
Nov 8, 2020·Annual Review of Physiology·Bhagirath Chaurasia, Scott A Summers
Apr 22, 2021·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Arturo Vega-BeyhartFelicia A Hanzu
Apr 1, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Daniel A PeñalvaMaria L Fanani

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