Implication of the differential roles of metallothionein 1 and 2 isoforms in the liver of rats as determined by polyacrylamide-coated capillary zone electrophoresis

Biochemical and Biophysical Research Communications
Shunsuke NakamuraH Sakurai

Abstract

Metallothioneins (MTs), determined by polyacrylamide-coated capillary zone electrophoresis (CZE), coincided well with those described by enzyme-linked immunosorbent assay. By using CZE, MT isoforms 1 (MT-1) and 2 (MT-2) were well separated and determined in the liver cytosol of LEC rats and Wistar rats administered CdCl(2). The total concentrations of MTs in the liver cytosol of LEC rats increased age-dependently as 1.0, 2.1, and 7.2mg/g wet weight of the liver at the age of 5, 10, and 15 weeks, respectively, and those of Wistar rats that had received daily CdCl(2) also increased with time of CdCl(2) as 0.5 and 1.2mg/g wet weight of the liver for 3 and 6 consecutive administration days, respectively. The MT-1/MT-2 ratio in the liver cytosol of LEC rats decreased age-dependently as 1.75, 1.49, and 0.76 at the age of 5, 10, and 15 weeks, respectively. In contrast, that of Wistar rats increased with time of exposure to the metal ion CdCl(2) as 1.1 and 1.6 for 3 and 6 administration days, respectively. Copper accumulation in the liver of LEC rats has already been reported. The present results indicated that the mechanism of the induction of MT synthesis differs between LEC rats, who lack ATP7B, and Wistar rats, who were given a tox...Continue Reading

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Citations

Jun 30, 2005·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Pham T N DiepCatherine Jumarie
Aug 9, 2013·Metallomics : Integrated Biometal Science·Ester ArtellsSílvia Atrian
Sep 8, 2011·Journal of Separation Science·Marta ZalewskaHalina Milnerowicz
Dec 5, 2009·Electrophoresis·Sona KrizkovaRene Kizek
Mar 30, 2010·Electrophoresis·Ana Valéria Colnaghi SimionatoMarina Franco Maggi Tavares
Nov 25, 2005·Electrophoresis·Vladislav Dolník
May 17, 2017·Proceedings of the National Academy of Sciences of the United States of America·Cheol-Hee ShinSung-Gil Chi

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