PMID: 9165086Mar 1, 1997Paper

Implications for the catalytic mechanism of the vanadium-containing enzyme chloroperoxidase from the fungus Curvularia inaequalis by X-ray structures of the native and peroxide form

Biological Chemistry
A MesserschmidtR Wever

Abstract

Implications for the catalytic mechanism of the vanadium-containing chloroperoxidase from the fungus Curvularia inaequalis have been obtained from the crystal structures of the native and peroxide forms of the enzyme. The X-ray structures have been solved by difference Fourier techniques using the atomic model of the azide chloroperoxidase complex. The 2.03 A crystal structure (R = 19.7%) of the native enzyme reveals the geometry of the intact catalytic vanadium center. The vanadium is coordinated by four non-protein oxygen atoms and one nitrogen (NE2) atom from histidine 496 in a trigonal bipyramidal fashion. Three oxygens are in the equatorial plane and the fourth oxygen and the nitrogen are at the apexes of the bipyramid. In the 2.24 A crystal structure (R = 17.7%) of the peroxide derivate the peroxide is bound to the vanadium in an eta2-fashion after the release of the apical oxygen ligand. The vanadium is coordinated also by 4 non-protein oxygen atoms and one nitrogen (NE2) from histidine 496. The coordination geometry around the vanadium is that of a distorted tetragonal pyramid with the two peroxide oxygens, one oxygen and the nitrogen in the basal plane and one oxygen in the apical position. A mechanism for the catalyti...Continue Reading

References

Jan 1, 1968·Advances in Protein Chemistry·G N Ramachandran, V Sasisekharan
Oct 1, 1994·European Journal of Biochemistry·J W Van SchijndelR Wever
Feb 13, 1993·Biochimica Et Biophysica Acta·J W van SchijndelR Wever
Jan 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·A Messerschmidt, R Wever

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Citations

Apr 14, 2009·The Journal of Biological Chemistry·Jaclyn M Winter, Bradley S Moore
Jun 9, 2009·Journal of Molecular Biology·Xiaofeng ZhuJames H Naismith
Apr 15, 2008·Bioresource Technology·Selvia Kurniawati, James A Nicell
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Jan 21, 2017·Chemical Reviews·Vinayak AgarwalBradley S Moore
May 4, 2017·Chemical Reviews·Jonathan LathamJason Micklefield
May 27, 2017·Chemical Communications : Chem Comm·Jia Jia DongFrank Hollmann
Jun 10, 2008·Journal of Inorganic Biochemistry·Yuan Zhang, José A Gascón
Mar 31, 2015·Journal of Lipid Research·Xiaoyun TangDavid N Brindley
Jan 23, 2015·Metallomics : Integrated Biometal Science·Dieter Rehder
Jul 15, 2004·Dalton Transactions : an International Journal of Inorganic Chemistry·Marian Casny, Dieter Rehder
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Aug 29, 2006·Chemical Society Reviews·James H Naismith
May 1, 2007·Theoretical Chemistry Accounts·Giorgio MoroGiuseppe Zampella
Mar 5, 2005·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Carole ColinPhilippe Potin
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Jan 6, 2009·Dalton Transactions : an International Journal of Inorganic Chemistry·Jana ChrappováJaromír Marek
Apr 25, 2012·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Daniele SannaEugenio Garribba
Mar 24, 2012·Dalton Transactions : an International Journal of Inorganic Chemistry·Tapan K SiKalyan K Mukherjea
Mar 12, 2008·Acta Biochimica Et Biophysica Sinica·Xianping Chen, Karl-Heinz van Pée
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