Implications for the evolution of eukaryotic amino-terminal acetyltransferase (NAT) enzymes from the structure of an archaeal ortholog

Proceedings of the National Academy of Sciences of the United States of America
Glen Liszczak, Ronen Marmorstein

Abstract

Amino-terminal acetylation is a ubiquitous modification in eukaryotes that is involved in a growing number of biological processes. There are six known eukaryotic amino-terminal acetyltransferases (NATs), which are differentiated from one another on the basis of substrate specificity. To date, two eukaryotic NATs, NatA and NatE, have been structurally characterized, of which NatA will acetylate the α-amino group of a number of nonmethionine amino-terminal residue substrates such as serine; NatE requires a substrate amino-terminal methionine residue for activity. Interestingly, these two NATs use different catalytic strategies to accomplish substrate-specific acetylation. In archaea, where this modification is less prevalent, only one NAT enzyme has been identified. Surprisingly, this enzyme is able to acetylate NatA and NatE substrates and is believed to represent an ancestral NAT variant from which the eukaryotic NAT machinery evolved. To gain insight into the evolution of NAT enzymes, we determined the X-ray crystal structure of an archaeal NAT from Sulfolobus solfataricus (ssNAT). Through the use of mutagenesis and kinetic analysis, we show that the active site of ssNAT represents a hybrid of the NatA and NatE active sites, ...Continue Reading

References

Mar 29, 2001·The Journal of Biological Chemistry·B Polevoda, F Sherman
Jul 5, 2001·Cellular and Molecular Life Sciences : CMLS·R Marmorstein
Aug 2, 2003·Biochemical and Biophysical Research Communications·Bogdan Polevoda, Fred Sherman
Aug 14, 2003·The Journal of Biological Chemistry·Ok-kyu SongRolf Sternglanz
Jun 2, 2007·Journal of Cellular Biochemistry·Bogdan PolevodaFred Sherman
Sep 26, 2008·Journal of Proteome Research·P Aaron KirklandJulie A Maupin-Furlow
May 8, 2009·Proceedings of the National Academy of Sciences of the United States of America·Thomas ArnesenKris Gevaert
Sep 12, 2009·The Journal of Biological Chemistry·Rune EvjenthJohan R Lillehaug
Jan 30, 2010·Science·Cheol-Sang HwangAlexander Varshavsky
Oct 5, 2010·Archaea : an International Microbiological Journal·Jörg Soppa
Jun 10, 2011·PLoS Biology·Gabriella M A ForteColin J Stirling
Aug 6, 2013·Nature Structural & Molecular Biology·Glen LiszczakRonen Marmorstein

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Citations

Feb 11, 2016·Scientific Reports·Om Singh RathoreRui Gonçalo Martinho
Dec 3, 2014·Biochimie·Carmela GiglioneThierry Meinnel
Aug 9, 2016·Trends in Biochemical Sciences·Henriette AksnesThomas Arnesen
Jun 12, 2019·Molecular & Cellular Proteomics : MCP·Jingjing CaoLi Huang
Jul 8, 2020·Molecular Systems Biology·Willy V BienvenutIris Finkemeier
Jan 13, 2016·The Journal of Biological Chemistry·Robert S MaginRonen Marmorstein
Mar 28, 2018·Proceedings of the National Academy of Sciences of the United States of America·Marianne GorisThomas Arnesen
May 13, 2020·Applied Biochemistry and Biotechnology·Yulia S LaptevaSergei E Permyakov
Dec 29, 2020·PLoS Computational Biology·Bojan KrtenicNathalie Reuter
Mar 25, 2020·Computational and Structural Biotechnology Journal·Angèle AbboudNathalie Reuter
Aug 1, 2019·Molecular & Cellular Proteomics : MCP·Jingjing CaoLi Huang
Apr 17, 2017·Biochemical and Biophysical Research Communications·Sungwook Shin, Jungwoo Choe
May 22, 2021·Structure·Sunbin DengRonen Marmorstein

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