Implications of mitochondrial network organization in mitochondrial stress signalling in NARP cybrid and Rho0 cells

Scientific Reports
Jarosław WalczakJoanna Szczepanowska

Abstract

Mitochondrial dysfunctions lead to the generation of signalling mediators that influence the fate of that organelle. Mitochondrial dynamics and their positioning within the cell are important elements of mitochondria-nucleus communication. The aim of this project was to examine whether mitochondrial shape, distribution and fusion/fission proteins are involved in the mitochondrial stress response in a cellular model subjected to specifically designed chronic mitochondrial stress: WT human osteosarcoma cells as controls, NARP cybrid cells as mild chronic stress and Rho0 as severe chronic stress. We characterized mitochondrial distribution in these cells using confocal microscopy and evaluated the level of proteins directly involved in the mitochondrial dynamics and their regulation. We found that the organization of mitochondria within the cell is correlated with changes in the levels of proteins involved in mitochondrial dynamics and proteins responsible for regulation of this process. Induction of the autophagy/mitophagy process, which is crucial for cellular homeostasis under stress conditions was also shown. It seems that mitochondrial shape and organization within the cell are implicated in retrograde signalling in chronic m...Continue Reading

References

Jan 1, 1996·Methods in Enzymology·M P King, G Attardi
Feb 19, 2004·The Journal of Biological Chemistry·Lorena GriparicAlexander M van der Bliek
Oct 2, 2004·The Journal of Biological Chemistry·Rie SugiokaYoshihide Tsujimoto
Oct 29, 2004·Proceedings of the National Academy of Sciences of the United States of America·Sara CipolatLuca Scorrano
May 19, 2005·The Journal of Biological Chemistry·Hsiuchen ChenDavid C Chan
Jun 25, 2005·Current Opinion in Cell Biology·Robert E Jensen
Jun 17, 2006·The EMBO Journal·Naotada IshiharaKatsuyoshi Mihara
Sep 28, 2006·The Journal of Biological Chemistry·Stéphane Duvezin-CaubetAndreas S Reichert
Feb 16, 2007·The Journal of Biological Chemistry·Naoko TaguchiKatsuyoshi Mihara
Dec 27, 2008·Cell·Carsten Merkwirth, Thomas Langer
Feb 10, 2009·Methods in Enzymology·Motoni Kadowaki, Md Razaul Karim
May 30, 2009·Molecular Biology of the Cell·Zhiyin SongDavid C Chan
Dec 30, 2009·The Journal of Cell Biology·Brian HeadAlexander M van der Bliek
Mar 26, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Albert MiskoRobert H Baloh
Apr 7, 2011·The EMBO Journal·Martin Graef, Jodi Nunnari
May 10, 2011·The International Journal of Biochemistry & Cell Biology·Marta WojewodaJoanna Szczepanowska
Feb 11, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jalees RehmanStephen L Archer
Oct 4, 2012·Free Radical Biology & Medicine·Chad A GallowayYisang Yoon
Nov 14, 2012·Biochimica Et Biophysica Acta·M Lamar SeibenhenerMarie W Wooten
Jan 4, 2013·Molecular Biology of the Cell·Oliver C LosónDavid C Chan
Feb 1, 2013·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Martin PicardYan Burelle
Nov 2, 2013·Cell Death & Disease·Y RuanZ Song
Nov 8, 2013·Molecular Biology of the Cell·Qinfang ShenAlexander M van der Bliek
Mar 13, 2014·The Journal of Cell Biology·Ruchika AnandThomas Langer
May 3, 2014·Molecular Biology of the Cell·Patrick J MacdonaldRajesh Ramachandran
Feb 18, 2015·The Journal of Cell Biology·Arnaud MourierNils-Göran Larsson

❮ Previous
Next ❯

Citations

Aug 5, 2020·Nature Immunology·Takahiro YamazakiLorenzo Galluzzi
Mar 17, 2021·Investigative Ophthalmology & Visual Science·Jessica M SkeieMark A Greiner
Sep 21, 2021·The EMBO Journal·Sergio Guerrero-CastilloSusanne Arnold

❮ Previous
Next ❯

Methods Mentioned

BETA
GTPases
GTPase
confocal microscopy

Software Mentioned

ImageJ
Odyssey
Zeiss LSM780

Related Concepts

Related Feeds

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms