Implications of secondary structure prediction and amino acid sequence comparison of class I and class II phosphoribosyl diphosphate synthases on catalysis, regulation, and quaternary structure

Protein Science : a Publication of the Protein Society
B N Krath, B Hove-Jensen

Abstract

Spinach 5-phospho-D-ribosyl alpha-1-diphosphate (PRPP) synthase isozyme 4 was synthesized in Escherichia coli and purified to near homogeneity. The activity of the enzyme is independent of P(i); it is inhibited by ADP in a competitive manner, indicating a lack of an allosteric site; and it accepts ATP, dATP, GTP, CTP, and UTP as diphosphoryl donors. All of these properties are characteristic for class II PRPP synthases. K(m) values for ATP and ribose 5-phosphate are 77 and 48 microM, respectively. Gel filtration reveals a molecular mass of the native enzyme of approximately 110 kD, which is consistent with a homotrimer. Secondary structure prediction shows that spinach PRPP synthase isozyme 4 has a general folding similar to that of Bacillus subtilis class I PRPP synthase, for which the three-dimensional structure has been solved, as the position and extent of helices and beta-sheets of the two enzymes are essentially conserved. Amino acid sequence comparison reveals that residues of class I PRPP synthases interacting with allosteric inhibitors are not conserved in class II PRPP synthases. Similarly, residues important for oligomerization of the B. subtilis enzyme show little conservation in the spinach enzyme. In contrast, res...Continue Reading

References

Nov 1, 1989·Molecular Microbiology·B Hove-Jensen
Sep 1, 1989·Molecular & General Genetics : MGG·D NilssonK Arnvig
Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Oct 14, 1970·Journal of Molecular Biology·M Mandel, A Higa
Jan 1, 1995·Advances in Enzyme Regulation·M TatibanaI Ahmad
Jul 20, 1993·Journal of Molecular Biology·B Rost, C Sander
Feb 3, 1999·Bioinformatics·K KarplusR Hughey
Feb 3, 1999·Bioinformatics·J A CuffG J Barton
Feb 19, 2000·Protein Engineering·R D KingD Page
Jul 13, 2000·Protein Science : a Publication of the Protein Society·M Ouali, R D King

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Citations

Jun 6, 2017·Acta Crystallographica. Section F, Structural Biology Communications·Vladimir I TimofeevRoman S Esipov
Nov 1, 2016·Extremophiles : Life Under Extreme Conditions·Tahira BibiMuhammad Akhtar
Jan 17, 2019·BMC Structural Biology·Weijie ZhouJarrod B French
Sep 1, 2008·EcoSal Plus·Kaj Frank JensenMartin WillemoËs

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