PMID: 8606365Nov 1, 1995Paper

Import of phosphatidylethanolamine for the assembly of rat brain mitochondrial membranes

The Journal of Membrane Biology
O Camici, L Corazzi

Abstract

Mitochondria can synthesize phosphatidyl-ethanolamine (PE) through phosphatidylserine decarboxylase (PS decarboxylase) activity or can import this lipid from the endoplasmic reticulum. In this work, we studied the factors influencing the import of PE in brain mitochondria and its utilization for the assembly of mitochondrial membranes. Incubation of rat brain homogenate with [1-3H]ethanolamine resulted in the synthesis and distribution of 3H-PE to subcellular fractions. Twenty-one percent of labeled PE was recovered in purified mitochondria. The import of PE in mitochondria was studied in a reconstituted system made of microsomes (donor particles) and purified mitochondria (acceptor particles). Ca2+ and nonspecific lipid transfer protein purified from liver tissue (nsL-TP) enhanced the translocation process. 3H-PE synthesized in membrane associated to mitochondria (MAM) could also translocate to mitochondria in the reconstituted system. Exposure of mitochondria to trinitrobenzensulfonic acid (TNBS) resulted in the reaction of more than 60% of 3H-PE imported from endoplasmic reticulum and of about 25% of 14C-PE produced in mitochondria by decarboxylation of 14C-PS. Moreover, the removal of the outer mitochondrial membrane by dig...Continue Reading

Citations

Aug 26, 2009·The International Journal of Biochemistry & Cell Biology·Magdalena LebiedzinskaMariusz R Wieckowski
Sep 8, 2010·Developmental Disabilities Research Reviews·Shane L ReaMarni J Falk
Nov 20, 2012·Biochimica Et Biophysica Acta·Ville KainuPentti Somerharju
Jul 6, 2014·Progress in Lipid Research·Hee-Yong KimArthur A Spector
Jan 13, 2015·Biochimica Et Biophysica Acta·Valentina RealiFederica Di Sano
Apr 11, 2007·FEMS Yeast Research·Andreas PerktoldGünther Zellnig
Oct 24, 2002·The Journal of Biological Chemistry·Marc M SchumacherDennis R Voelker
Jul 3, 2004·The Journal of Biological Chemistry·Lara MacchioniGianfrancesco Goracci
Jun 13, 1998·Progress in Lipid Research·G Daum, J E Vance

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