Importance of an immunodominant surface-exposed loop on outer membrane protein P2 of nontypeable Haemophilus influenzae.

Infection and Immunity
K Yi, Timothy F Murphy

Abstract

Nontypeable Haemophilus influenzae (NTHI) frequently causes recurrent infections of the respiratory tract in humans. Previous indirect evidence suggested that a strain-specific immune response occurs following infection and that this immune response is directed at an immunodominant epitope on the bacterial surface. To test this hypothesis, mice and rabbits were immunized with whole cells of a strain of NTHI and the antiserum was characterized to identify the antigens to which antibodies were directed. All animals made a prominent antibody response to the loop 5 region of the P2 molecule, which is the major outer membrane protein. Rabbit serum showed complement-dependent bactericidal activity. Adsorption of the immune serum with the loop 5 fusion peptide removed bactericidal activity and also abolished reactivity to P2 detected by an immunoblot assay, an enzyme-linked immunosorbent assay, and a radioimmunoprecipitation assay. These data indicate that immunization with whole cells of NTHI results in a prominent antibody response which is directed at epitopes on the loop 5 region of the P2 molecule. Thus, a strain-specific immune response to NTHI occurs as a result of the expression of an immunodominant epitope on the P2 molecule.

References

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Citations

Jun 9, 1998·Annals of the New York Academy of Sciences·T F Murphy, K Yi
Feb 18, 2003·American Journal of Respiratory and Critical Care Medicine·Timothy F Murphy
Dec 20, 2002·The New England Journal of Medicine·Amar Kureishi
Nov 5, 2003·American Journal of Respiratory and Critical Care Medicine·Sanjay SethiTimothy F Murphy
May 23, 2000·The Pediatric Infectious Disease Journal·T F Murphy
Aug 7, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Laura A Novotny, Lauren O Bakaletz
Feb 9, 2018·Expert Review of Anti-infective Therapy·Karen L OsmanStuart C Clarke
Oct 24, 1998·Infection and Immunity·J R Gilsdorf
May 3, 2005·Veterinary Microbiology·Md Shahidur R KhanYuichi Tagawa

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