Importance of assessing CK19 immunostaining in core biopsies in patients subjected to sentinel node study by OSNA.

Virchows Archiv : an International Journal of Pathology
Felip VilardellXavier Matias-Guiu

Abstract

Analysis of sentinel lymph node (SLN) by means of One-Step Nucleic Acid Amplification (OSNA) is being used increasingly as a very sensitive and quick method for intraoperative axillary staging in patients with breast cancer. This molecular diagnostic assay detects the expression level of cytokeratin 19 (CK19), a luminal epithelial cell marker broadly expressed in most breast carcinomas and not normally found in lymph nodes. Almost all breast cancers express this cytoskeleton protein, but some breast tumors have been found to lose the expression of CK19. CK19 immunostaining in core biopsies has been recommended in selecting patients eligible for OSNA analysis because SLNs with metastatic involvement by CK19-negative breast cancers may result in a false negative result by OSNA. However, the real frequency of CK19-negative breast cancer has to be elucidated. In this study, we have assessed the frequency and molecular profile of CK19-negative breast carcinomas in three series of cases. The first is a prospective series of 197 breast carcinomas, 111 of which were subjected to SLN evaluation by OSNA. The second is a retrospective series of 41 triple-negative (TN) breast carcinomas, and the third is a retrospective series of 68 breast...Continue Reading

References

May 20, 1999·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·J M HarveyD C Allred
Aug 30, 2000·Nature·C M PerouD Botstein
May 16, 2002·Histopathology·P G Chu, L M Weiss
May 14, 2004·The Journal of Pathology·Dalia M Abd El-RehimIan O Ellis
Aug 26, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Torsten O NielsenCharles M Perou
Aug 19, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Masahiko TsujimotoNariaki Matsuura
Sep 15, 2007·International Journal of Radiation Oncology, Biology, Physics·Rahul R ParikhBruce G Haffty
Mar 8, 2008·International Journal of Cancer. Journal International Du Cancer·Mike VisserChris J L M Meijer
May 20, 2008·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Emad A RakhaIan O Ellis
Jan 13, 2011·Cancer·Sheldon FeldmanUNKNOWN US One Step Nucleic Acid Amplification Clinical Study Group
Jun 10, 2011·The British Journal of Surgery·K L SnookUNKNOWN OSNA Study Group
Jun 29, 2011·Annals of Oncology : Official Journal of the European Society for Medical Oncology·A GoldhirschUNKNOWN Panel members

❮ Previous
Next ❯

Citations

Jun 15, 2013·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Muhammad T IdreesLiang Cheng
Dec 24, 2014·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·Oscar GonzálezJosé María Balibrea
Aug 1, 2012·Cytopathology : Official Journal of the British Society for Clinical Cytology·C Boyd, D P Boyle
Mar 17, 2016·European Journal of Surgical Oncology : the Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·S M BanerjeeM R S Keshtgar
Aug 10, 2012·Breast Cancer : the Journal of the Japanese Breast Cancer Society·Yasuhiro Tamaki
Sep 18, 2013·The Breast : Official Journal of the European Society of Mastology·Kenjiro JimboHitoshi Tsuda
Nov 28, 2014·Virchows Archiv : an International Journal of Pathology·Xiaopeng HaoChengze Yu
Dec 29, 2015·Histopathology·Begoña VieitesJosé Palacios
Jun 26, 2013·International Journal of Molecular Sciences·Salma Al-RamadhaniVasi Sundaresan
Nov 16, 2012·Virchows Archiv : an International Journal of Pathology·Felip Vilardell, X Matias-Guiu
Jan 1, 2012·Cancers·Mamiko FujisueNobuyuki Arima
Oct 25, 2012·Virchows Archiv : an International Journal of Pathology·Dionysios D RemoundosGiles H Cunnick

❮ Previous
Next ❯

Methods Mentioned

BETA
Nucleic Acid Amplification
biopsy
biopsies

Software Mentioned

Statistical Package for Social Science ( SPSS )
OSNA

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.