Importance of cholesterol for infection of cells by transmissible gastroenteritis virus.

Virus Research
Xiaofeng RenGeorg Herrler

Abstract

In this study, we addressed the question whether cholesterol is important for transmissible gastroenteritis virus (TGEV), a porcine coronavirus, in the initiation of an infection. We found that cholesterol depletion from the cellular membrane by methyl-beta-cyclodextrin (MbetaCD) significantly impaired the efficiency of TGEV infection. Infectivity was also reduced after depleting cholesterol from the viral envelope. This finding is surprising because coronaviruses bud from a pre-Golgi compartment which is expected to be low in cholesterol compared to the plasma membrane. Addition of exogenous cholesterol resulted in a restoration of the infectivity confirming our conclusion that efficient TGEV infection requires cholesterol in both the viral and the cellular membranes. Our data raise the possibility that the viral and cellular proteins involved in the entry process may be associated with cholesterol-rich membrane microdomains.

References

Dec 1, 1988·The Journal of General Virology·W SpaanM C Horzinek
Oct 15, 2003·Journal of Virology·Christel Schwegmann-WesselsGeorg Herrler
Mar 3, 2004·Journal of Virology·Edward B Thorp, Thomas M Gallagher
Apr 10, 2004·Science·Alicia E Smith, Ari Helenius
Jul 29, 2004·Journal of Virology·Ryuji NomuraToyoshi Fujimoto
Nov 27, 2004·Journal of Cell Science·Sebastian Schuck, Kai Simons
Apr 11, 2006·The Journal of General Virology·Christine WinterGeorg Herrler
Aug 2, 2006·Biological & Pharmaceutical Bulletin·Takashi Suzuki, Yasuo Suzuki
Feb 3, 2007·Journal of Virology·Heidi ImhoffLudwig Haas

❮ Previous
Next ❯

Citations

Jun 3, 2014·Virology Journal·Jin CuiShoujun Li
Aug 19, 2011·Avian Pathology : Journal of the W.V.P.A·Jiechao YinXiaofeng Ren
Apr 23, 2013·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Qiong ZhaoXiaofeng Ren
Oct 12, 2013·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Liyan CaoXiaofeng Ren
Oct 1, 2010·Biotechnology Letters·Xiaofeng RenYong-Suk Jang
Jul 14, 2010·Current Microbiology·Xiaofeng RenGeorg Herrler
Jun 11, 2015·Veterinary Microbiology·Graham BrogdenDieter Steinhagen
Mar 23, 2013·Virus Research·Ana C Carro, Elsa B Damonte
Oct 31, 2015·Archives of Virology·Tomomi TakanoTsutomu Hohdatsu
Oct 18, 2015·Virus Research·Annamaria Pratelli, Valeriana Colao
Nov 9, 2014·The Journal of General Virology·Annamaria Pratelli, Valeriana Colao
May 11, 2013·Acta Veterinaria Hungarica·Wei YangXiaofeng Ren
Sep 9, 2017·Archives of Virology·Ji Hyun Jeon, Changhee Lee
Jul 28, 2018·Archives of Virology·Ji Hyun Jeon, Changhee Lee
Feb 6, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jian WangFei Liu
Apr 7, 2021·QJM : Monthly Journal of the Association of Physicians·M ZuinL Roncon
Aug 7, 2017·Antiviral Research·Tomomi TakanoTsutomu Hohdatsu
Jun 22, 2021·Biochimie·Stéphane OrlowskiEric Bruckert

❮ Previous
Next ❯

Methods Mentioned

BETA
FCS
Assay

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.