Importance of cholinesterase kinetic parameters in environmental monitoring using estuarine fish

Chemosphere
V TortelliJosé M Monserrat

Abstract

The aim of the present study was to determine the kinetic parameters and cholinesterase (ChE) activity in two teleost fish, the white mouth croaker Micropogonias furnieri (Scianidae) and the Madamango sea catfish Cathorops spixii (Ariidae), to verify their suitability as sentinel species of aquatic pollution by anticholinergic compounds. Individuals of each species were captured in one reference and one polluted site in the Southern Brazilian coast. Brain tissue was used as enzyme source. Inhibition kinetic parameters indicated that ChE from C. spixii collected at the reference site showed more affinity (Ka) for eserine that from those collected at the polluted site. The opposite was observed for the carbamylation constants (Kc). Thus, similar inhibition constants (Ki) were observed. M. furnieri brain showed an extremely low sensitivity to in vitro inhibition by eserine, indicating that it is not a suitable biomarker to be employed in environmental monitoring of anticholinergic compounds. Results from the present study also point to the need for considering kinetic analysis when cholinesterase activity is employed as a biomarker.

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Citations

Apr 27, 2013·Environmental Toxicology and Pharmacology·Erol Capkin, Ilhan Altinok
Feb 28, 2009·Ecotoxicology and Environmental Safety·Stefano BonacciSilvano Focardi
Apr 4, 2008·Ecotoxicology and Environmental Safety·Alpuche-Gual Leticia, Gold-Bouchot Gerardo
Oct 19, 2006·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·José M MonserratAdalto Bianchini
Jul 28, 2013·Ecotoxicology and Environmental Safety·Gabriela Rodríguez-FuentesKaren Luna-Ramírez
Oct 13, 2016·Bulletin of Environmental Contamination and Toxicology·Gabriela Rodríguez-FuentesEsperanza Hernández-Márquez
Nov 30, 2020·The Science of the Total Environment·Manuela S SantanaMaritana Mela Prodocimo
Nov 23, 2021·Environmental Science and Pollution Research International·Muryllo Santos CastroRicardo Luvizotto-Santos

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