Importance of complement source in measuring meningococcal bactericidal titers

Clinical and Diagnostic Laboratory Immunology
G F SantosDan M Granoff

Abstract

Complement-mediated bactericidal antibodies in serum confer protection against meningococcal disease. The minimum protective titer is estimated to be between 1:4 and 1:8 when measured by the Goldschneider assay performed with human complement, the assay used in the 1960s to establish the correlation between bactericidal antibodies and protection. A more recently described bactericidal assay standardized by an international consortium uses rabbit complement, which is known to augment bactericidal titers. To define a protective titer measured by the standardized assay, we compared bactericidal titers against serogroup C strains measured by this assay to titers measured by the assay described by Goldschneider et al. A titer of > or =1:128 measured by the standardized assay was needed to predict with > or =80% certainty a positive titer of > or =1:4 as measured by the Goldschneider assay. However, the majority of samples with titers of 1:4 measured by the Goldschneider assay had titers of <1:128 when measured by the standardized assay. Therefore, by the results of the standardized assay such persons would be falsely categorized as being susceptible to disease. In conclusion, high bactericidal titers measured with the standardized a...Continue Reading

References

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Citations

Oct 17, 2002·FEMS Immunology and Medical Microbiology·Clementien Vermont, Germie van den Dobbelsteen
Aug 26, 2003·Biologicals : Journal of the International Association of Biological Standardization·Tamara RodríguezOliver Pérez
Jan 5, 2006·The Journal of Experimental Medicine·Concetta BeninatiGiuseppe Teti
Feb 11, 2010·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Dan M Granoff
Nov 13, 2012·PloS One·Colette M O'ShaughnessyCalman A Maclennan
Sep 2, 2004·Paediatric Drugs·Jens U Rüggeberg, Andrew J Pollard
Jan 5, 2002·Expert Opinion on Biological Therapy·Raman Lakshman, Adam Finn
Mar 17, 2007·Pediatric Blood & Cancer·Joyce W YuBruce D Mazer
Mar 15, 2013·Journal of Immunological Methods·Charlotte BrookesStephen Taylor
Sep 16, 2009·Vaccine·Gretel SardiñasDaniel Yero
Aug 3, 2012·Human Vaccines & Immunotherapeutics·Kim S Erlich, Blaise L Congeni
Apr 1, 2014·The Lancet Infectious Diseases·Sophie M Andrews, Andrew J Pollard
Apr 24, 2009·Vaccine·Jo Anne Welsch, Sanjay Ram
May 21, 2011·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·James B WingRobert C Read

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