Importance of creatine kinase activity for functional recovery of myocardium after ischemia-reperfusion challenge

Journal of Cardiovascular Pharmacology
Patricia RodriguezS Barrigon

Abstract

To define the relation between the phosphoryl transfer via creatine kinase and the ability to recover from an ischemia-reperfusion challenge, the authors chemically inhibited creatine kinase activity with iodoacetamide (IAm) and then measured myocardial recovery after 2, 10, or 30 min of global ischemia followed by 30 min of reperfusion in the isolated, arterially perfused interventricular septa of the rabbit heart. During normoxia, IAm (0.5 M perfused for 15 min) did not by itself modify developed tension, maximal rate of tension development, or resting tension. In ischemia, IAm pretreatment increased the rate of developed tension loss and highly diminished developed tension recovery after reperfusion for all the ischemia periods tested. Moreover, IAm significantly enhanced the maximal increase in the resting tension induced by 10 or 30 min of ischemia plus reperfusion. Lactate dehydrogenase activity in reperfusion was also significantly increased over untreated septa. On the basis of the present results, the authors suggest that the aggravating effects exhibited by IAm on the ischemic myocardium are compatible with its creatine kinase inhibition properties and that creatine kinase activity is essential for full recovery from ...Continue Reading

References

Aug 14, 2012·American Journal of Physiology. Heart and Circulatory Physiology·Ashwin AkkiRobert G Weiss
Apr 20, 2011·Expert Review of Proteomics·Hyoung Kyu KimJin Han

Citations

Jun 1, 1992·Circulation Research·Richmond W JeremyL C Becker
Aug 1, 1991·The Journal of Clinical Investigation·J A Lee, D G Allen
Oct 1, 1994·Journal of Cardiovascular Pharmacology·E F du Toit, L H Opie
Aug 25, 1995·The Journal of Biological Chemistry·R Ventura-ClapierV I Veksler
May 1, 1993·The Journal of Surgical Research·M B MitchellA H Harken
Mar 1, 1993·Journal of Molecular and Cellular Cardiology·Richmond W JeremyL C Becker
Feb 15, 1997·The Journal of Clinical Investigation·R TianJ S Ingwall
Nov 20, 1998·Journal of Cardiovascular Pharmacology·M AvellanalS Barrigon

Related Concepts

Ischemia
Septa
Macro-Creatine Kinase
Myocardium
Myocardial Stunning
Myocardial Ischemia
Iodoacetone
Creatine Kinase Measurement
Chinchilla Rabbits
Creatine Kinase Activity

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