Importance of cytoskeletal elements in volume regulatory responses of trout hepatocytes

American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
Hannes L EbnerGerhard Krumschnabel

Abstract

The role of cytoskeletal elements in volume regulation was studied in trout hepatocytes by investigating changes in F-actin distribution during anisotonic exposure and assessing the impact of cytoskeleton disruption on volume regulatory responses. Hypotonic challenge caused a significant decrease in the ratio of cortical to cytoplasmic F-actin, whereas this ratio was unaffected in hypertonic saline. Disruption of microfilaments with cytochalasin B (CB) or cytochalasin D significantly slowed volume recovery following hypo- and hypertonic exposure in both attached and suspended cells. The decrease of net proton release and the intracellular acidification elicited by hypotonicity were unaltered by CB, whereas the increase of proton release in hypertonic saline was dramatically reduced. Because amiloride almost completely blocked the hypertonic increase of proton release and cytoskeleton disruption diminished the associated increase of intracellular pH (pH(i)), we suggest that F-actin disruption affected Na(+)/H(+) exchanger activity. In line with this, pH(i) recovery after an ammonium prepulse was significantly inhibited in CB-treated cells. The increase of cytosolic Na(+) under hypertonic conditions was not diminished but, rather...Continue Reading

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Related Concepts

Isoactin
Na(+)-K(+)-Exchanging ATPase
Body Water
Calcium
Cytochalasin B
Microtrabecular Lattice
Cytoplasmic Matrix
Hydrogen-Ion Concentration
Intracellular Fluid
Osmolality

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