Importance of cytoskeletal elements in volume regulatory responses of trout hepatocytes

American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
Hannes L EbnerGerhard Krumschnabel


The role of cytoskeletal elements in volume regulation was studied in trout hepatocytes by investigating changes in F-actin distribution during anisotonic exposure and assessing the impact of cytoskeleton disruption on volume regulatory responses. Hypotonic challenge caused a significant decrease in the ratio of cortical to cytoplasmic F-actin, whereas this ratio was unaffected in hypertonic saline. Disruption of microfilaments with cytochalasin B (CB) or cytochalasin D significantly slowed volume recovery following hypo- and hypertonic exposure in both attached and suspended cells. The decrease of net proton release and the intracellular acidification elicited by hypotonicity were unaltered by CB, whereas the increase of proton release in hypertonic saline was dramatically reduced. Because amiloride almost completely blocked the hypertonic increase of proton release and cytoskeleton disruption diminished the associated increase of intracellular pH (pH(i)), we suggest that F-actin disruption affected Na(+)/H(+) exchanger activity. In line with this, pH(i) recovery after an ammonium prepulse was significantly inhibited in CB-treated cells. The increase of cytosolic Na(+) under hypertonic conditions was not diminished but, rather...Continue Reading


Jan 1, 1976·The Journal of General Physiology·W F Boron, P De Weer
Nov 1, 1992·The American Journal of Physiology·N FrankiR M Hays
Mar 1, 1992·The American Journal of Physiology·F N ZiyadehA Kleinzeller
Dec 1, 1991·The American Journal of Physiology·K R HallowsP A Knauf
Jul 1, 1991·The American Journal of Physiology·M A LinshawA I Gotlieb
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D LipscombeR W Tsien
Feb 15, 1988·The Biochemical Journal·I H Madshus
Jan 1, 1986·The American Journal of Physiology·P J Walsh
Jan 1, 1985·The American Journal of Physiology·J K Foskett, K R Spring
Oct 1, 1995·The American Journal of Physiology·Bernd Pelster
Jan 1, 1995·International Review of Cytology·E K Hoffmann, P B Dunham
Apr 1, 1993·The Journal of Membrane Biology·M CornetH A Kolb
Nov 1, 1994·Experimental Physiology·F MichelP Saint-Marc
Apr 1, 1995·Journal of Cellular Physiology·G P DowneyC K Chan
Jun 1, 1994·The American Journal of Physiology·K J Bibby, C A McCulloch
Jan 1, 1994·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·D HäussingerC Stournaras
Feb 1, 1994·The Journal of Experimental Zoology·J W MillsB A Stanton
Oct 1, 1995·The Journal of Membrane Biology·I LevitanS S Garber
Jul 26, 1996·The Journal of Biological Chemistry·B K BerdievI I Ismailov
Mar 1, 1996·The American Journal of Physiology·Gerhard KrumschnabelW Wieser
Jan 1, 1997·Physiological Reviews·S WakabayashiJ Pouyssegur
Feb 11, 1998·Physiological Reviews·F LangD Häussinger
Apr 8, 1998·The American Journal of Physiology·J B MatthewsJ K Sicklick
Apr 16, 1998·International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity·J HagerP Froguel
Aug 5, 1998·The Journal of General Physiology·J AltamiranoF J Alvarez-Leefmans
Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·W P DubinskyS G Schultz
Aug 18, 1999·The Journal of Experimental Zoology·M FurimskyS F Perry
Sep 30, 1999·Experimental Cell Research·Stine F PedersenE K Hoffmann
Oct 9, 1999·The Journal of Biological Chemistry·K KurashimaS Grinstein
Aug 3, 2000·American Journal of Physiology. Heart and Circulatory Physiology·D B CowanF X McGowan
Dec 23, 2000·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·K SzásziA Kapus
Feb 17, 2001·General and Comparative Endocrinology·Gerhard KrumschnabelPablo J Schwarzbaum
Feb 24, 2001·Pflügers Archiv : European journal of physiology·F Wehner, H Tinel
Aug 21, 2001·Pflügers Archiv : European journal of physiology·C HougaardE K Hoffmann
Jan 25, 2002·Annual Review of Pharmacology and Toxicology·L K PutneyD L Barber
Mar 13, 2002·Pflügers Archiv : European journal of physiology·A J NichollC Garner
Mar 27, 2002·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Stine F PedersenJ W Mills
Sep 26, 2002·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Maria G LionettoTrifone Schettino
Nov 20, 2002·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Martin JakabMarkus Paulmichl
Dec 18, 2002·The Journal of Cell Biology·Sheryl P Denker, Diane L Barber
Dec 28, 2002·The Journal of Experimental Biology·M V EspeltPablo J Schwarzbaum
Mar 8, 2003·Osteoarthritis and Cartilage·Geoffrey R EricksonFarshid Guilak
Apr 11, 2003·Reviews of Physiology, Biochemistry and Pharmacology·F WehnerR K H Kinne
May 3, 2003·The Journal of Experimental Biology·Gerhard KrumschnabelPablo J Schwarzbaum
Jun 24, 2003·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·A TuominenM Nikinmaa
Sep 2, 2003·Biochimica Et Biophysica Acta·Nanna K JorgensenSøren-Peter Olesen
Jun 26, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Diego E PafundoPablo J Schwarzbaum
Aug 18, 2004·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Claudia ManzlReinhard Dallinger


Oct 24, 2008·Acta Physiologica·I H LambertStine F Pedersen
May 12, 2010·American Journal of Physiology. Cell Physiology·Susan Z HuaFrederick Sachs
Oct 13, 2010·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·C FaggioF Trischitta
Nov 28, 2008·European Journal of Cell Biology·Christopher BlaseJürgen Bereiter-Hahn
Sep 23, 2011·Journal of Cellular Biochemistry·L GaliziaP Ford
Feb 4, 2011·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Osvaldo CharaPablo J Schwarzbaum
Jul 15, 2015·Biochimica Et Biophysica Acta·Alexandra PlatonovaSergei N Orlov
Mar 12, 2013·Toxicology in Vitro : an International Journal Published in Association with BIBRA·A TorreC Faggio
Feb 3, 2006·American Journal of Physiology. Cell Physiology·Wen-Zhi LanCeredwyn E Hill
Jan 8, 2009·Physiological Reviews·E K HoffmannStine F Pedersen
Dec 29, 2020·Frontiers in Physiology·Bojan BožičGregor Gomišček
Sep 18, 2007·The Journal of Experimental Biology·Khaled H Ahmed, Bernd Pelster
Aug 5, 2006·The Journal of Experimental Biology·Khaled H AhmedGerhard Krumschnabel

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